TY - JOUR
T1 - Genomic cloning and characterization of a ricin gene from Ricinus Communis
AU - Halling, Kevin C.
AU - Halling, Amy C.
AU - Murray, Elizabeth E.
AU - Ladin, Beth F.
AU - Houston, L. L.
AU - Weaver, Robert F.
N1 - Funding Information:
ACKNOWLEDGMENTS This work was supported by a grant from Eli Lilly & Company, Indianapolis, Indiana.
PY - 1985/11/25
Y1 - 1985/11/25
N2 - A genomic clone that specifies a single poypeptide precursor for ricin, a toxic lectin of Ricinus communis (castor bean), was isolated, sequenced and S1 mapped. The gene encodes a 64 kDa precursor which contains, in the following order: a 24 or 35 amino acid signal peptide, the A chain, a 12 amino acid linker peptide, and the B chain. The 5′-end of the ricin mRNA maps approximately 35 bases upstream from the first methionine codon. Two putative TATA boxes and a possible CAAT box lie in the 5′-flanking region. Two possible polyadenylation signals are found in the 3′ flanking region. No introns were found, which is typical of other lectin genes that have been sequenced. Southern blot analysis suggests that the castor bean genome contains approximately six ricin-like genes.
AB - A genomic clone that specifies a single poypeptide precursor for ricin, a toxic lectin of Ricinus communis (castor bean), was isolated, sequenced and S1 mapped. The gene encodes a 64 kDa precursor which contains, in the following order: a 24 or 35 amino acid signal peptide, the A chain, a 12 amino acid linker peptide, and the B chain. The 5′-end of the ricin mRNA maps approximately 35 bases upstream from the first methionine codon. Two putative TATA boxes and a possible CAAT box lie in the 5′-flanking region. Two possible polyadenylation signals are found in the 3′ flanking region. No introns were found, which is typical of other lectin genes that have been sequenced. Southern blot analysis suggests that the castor bean genome contains approximately six ricin-like genes.
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U2 - 10.1093/nar/13.22.8019
DO - 10.1093/nar/13.22.8019
M3 - Article
C2 - 2999712
AN - SCOPUS:0022432538
SN - 0305-1048
VL - 13
SP - 8019
EP - 8033
JO - Nucleic acids research
JF - Nucleic acids research
IS - 22
ER -