Genomic approaches to deconstruct pluripotency

Yuin Han Loh, Lin Yang, Jimmy Chen Yang, Hu Li, James J. Collins, George Q. Daley

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Embryonic stem cells (ESCs) first derived from the inner cell mass of blastocyst-stage embryos have the unique capacity of indefinite self-renewal and potential to differentiate into all somatic cell types. Similar developmental potency can be achieved by reprogramming differentiated somatic cells into induced pluripotent stem cells (iPSCs). Both types of pluripotent stem cells provide great potential for fundamental studies of tissue differentiation, and hold promise for disease modeling, drug development, and regenerative medicine. Although much has been learned about the molecular mechanisms that underlie pluripotency in such cells, our understanding remains incomplete. A comprehensive understanding of ESCs and iPSCs requires the deconstruction of complex transcription regulatory networks, epigenetic mechanisms, and biochemical interactions critical for the maintenance of self-renewal and pluripotency. In this review, we will discuss recent advances gleaned from application of global "omics" techniques to dissect the molecular mechanisms that define the pluripotent state.

Original languageEnglish (US)
Pages (from-to)165-185
Number of pages21
JournalAnnual Review of Genomics and Human Genetics
Volume12
DOIs
StatePublished - Jul 13 2011

Keywords

  • DNA methylation
  • Epigenetics
  • Histone modifications
  • Pluripotent stem cells
  • Transcription regulation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Fingerprint Dive into the research topics of 'Genomic approaches to deconstruct pluripotency'. Together they form a unique fingerprint.

Cite this