TY - JOUR
T1 - Genome-wide resolution peripheral blood methylome profiling reveals signatures for cholestatic liver disease
AU - Moore, Raymond M.
AU - Sun, Zhifu
AU - Juran, Brian D.
AU - Lazaridis, Konstantinos N.
N1 - Funding Information:
This study was supported by RC2 DK118619 (KN Lazaridis) and the Chris M Carlos and Catharine Nicole Jockisch Carlos Endowment Fund in primary sclerosing cholangitis (PSC) (KN Lazaridis). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.
Publisher Copyright:
© 2020
PY - 2020/8
Y1 - 2020/8
N2 - Aim: To profile DNA methylation changes of primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC). Materials & methods: Patients with: PBC, PSC with inflammatory bowel disease (IBD), PSC without IBD, and age-, sex-matched controls were profiled for methylomes of peripheral blood by reduced representation bisulfite sequencing. Differentially methylated CpG (DMC) and differentially methylated region (DMR) were detected and compared. Results: We identified consistently altered DMCs and DMRs across diseases with involvement in key pathways. Many similarities noted between two subtypes of PSC, interestingly few existed between PBC and PSC. DMRs were highly enriched with transcription factor binding. Top DMC changes were validated in liver tissue of an independent cohort. Conclusion: Methylome profiling provides insights to PBC and PSC.
AB - Aim: To profile DNA methylation changes of primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC). Materials & methods: Patients with: PBC, PSC with inflammatory bowel disease (IBD), PSC without IBD, and age-, sex-matched controls were profiled for methylomes of peripheral blood by reduced representation bisulfite sequencing. Differentially methylated CpG (DMC) and differentially methylated region (DMR) were detected and compared. Results: We identified consistently altered DMCs and DMRs across diseases with involvement in key pathways. Many similarities noted between two subtypes of PSC, interestingly few existed between PBC and PSC. DMRs were highly enriched with transcription factor binding. Top DMC changes were validated in liver tissue of an independent cohort. Conclusion: Methylome profiling provides insights to PBC and PSC.
KW - differentially methylated CpG
KW - differentially methylated region
KW - methylation biomarker
KW - peripheral blood
KW - primary biliary cholangitis
KW - primary sclerosing cholangitis
KW - reduced representation bisulfite sequencing
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U2 - 10.2217/epi-2020-0048
DO - 10.2217/epi-2020-0048
M3 - Article
C2 - 32914644
AN - SCOPUS:85091324970
SN - 1750-1911
VL - 12
SP - 1363
EP - 1375
JO - Epigenomics
JF - Epigenomics
IS - 16
ER -