Genome-wide methylation profiling demonstrates hypermethylation in maternal leukocyte DNA in preeclamptic compared to normotensive pregnancies

Wendy M. White, Brian Brost, Zhifu D Sun, Carl Rose, Iasmina Craici, Steven J. Wagner, Stephen T Turner, Vesna D Garovic

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objective: To compare genome-wide methylation profiles in maternal leukocyte DNA between normotensive and preeclamptic pregnant women at delivery. Methods: Age, body mass index matched case-control comparison of methylation at 27,578 cytosine- guanine sites in 14,495 genes in maternal leukocyte DNA in women with preeclampsia (PE; n=14) and normotensive controls (n=14). Results: PE was associated with widespread differential methylation favoring hypermethylation. Pathway analysis identified the best matched process as a neuropeptide signaling pathway (p<10-5); best matched disease as eclampsia (p<9.97×10-20). Significantly differentially methylated genes (GRIN2b. GABRA1. PCDHB7, and BEX1) are associated with seizures. Conclusion: Altered maternal leukocyte DNA methylation is associated with PE at delivery, and differential methylation of certain neuronal genes may explain the risk for eclampsia.

Original languageEnglish (US)
Pages (from-to)257-269
Number of pages13
JournalHypertension in Pregnancy
Volume32
Issue number3
DOIs
StatePublished - Aug 2013

Fingerprint

Methylation
Leukocytes
Mothers
Genome
Pregnancy
Eclampsia
DNA
Genes
Cytosine
Guanine
DNA Methylation
Pre-Eclampsia
Neuropeptides
Pregnant Women
Seizures
Body Mass Index

Keywords

  • DNA methylation
  • Epigenetics
  • Leukocyte
  • Maternal
  • Preeclampsia
  • Pregnancy

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Internal Medicine

Cite this

Genome-wide methylation profiling demonstrates hypermethylation in maternal leukocyte DNA in preeclamptic compared to normotensive pregnancies. / White, Wendy M.; Brost, Brian; Sun, Zhifu D; Rose, Carl; Craici, Iasmina; Wagner, Steven J.; Turner, Stephen T; Garovic, Vesna D.

In: Hypertension in Pregnancy, Vol. 32, No. 3, 08.2013, p. 257-269.

Research output: Contribution to journalArticle

@article{fe1f92e8b1844b98bc77218ea9680439,
title = "Genome-wide methylation profiling demonstrates hypermethylation in maternal leukocyte DNA in preeclamptic compared to normotensive pregnancies",
abstract = "Objective: To compare genome-wide methylation profiles in maternal leukocyte DNA between normotensive and preeclamptic pregnant women at delivery. Methods: Age, body mass index matched case-control comparison of methylation at 27,578 cytosine- guanine sites in 14,495 genes in maternal leukocyte DNA in women with preeclampsia (PE; n=14) and normotensive controls (n=14). Results: PE was associated with widespread differential methylation favoring hypermethylation. Pathway analysis identified the best matched process as a neuropeptide signaling pathway (p<10-5); best matched disease as eclampsia (p<9.97×10-20). Significantly differentially methylated genes (GRIN2b. GABRA1. PCDHB7, and BEX1) are associated with seizures. Conclusion: Altered maternal leukocyte DNA methylation is associated with PE at delivery, and differential methylation of certain neuronal genes may explain the risk for eclampsia.",
keywords = "DNA methylation, Epigenetics, Leukocyte, Maternal, Preeclampsia, Pregnancy",
author = "White, {Wendy M.} and Brian Brost and Sun, {Zhifu D} and Carl Rose and Iasmina Craici and Wagner, {Steven J.} and Turner, {Stephen T} and Garovic, {Vesna D}",
year = "2013",
month = "8",
doi = "10.3109/10641955.2013.796970",
language = "English (US)",
volume = "32",
pages = "257--269",
journal = "Hypertension in Pregnancy",
issn = "1064-1955",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - Genome-wide methylation profiling demonstrates hypermethylation in maternal leukocyte DNA in preeclamptic compared to normotensive pregnancies

AU - White, Wendy M.

AU - Brost, Brian

AU - Sun, Zhifu D

AU - Rose, Carl

AU - Craici, Iasmina

AU - Wagner, Steven J.

AU - Turner, Stephen T

AU - Garovic, Vesna D

PY - 2013/8

Y1 - 2013/8

N2 - Objective: To compare genome-wide methylation profiles in maternal leukocyte DNA between normotensive and preeclamptic pregnant women at delivery. Methods: Age, body mass index matched case-control comparison of methylation at 27,578 cytosine- guanine sites in 14,495 genes in maternal leukocyte DNA in women with preeclampsia (PE; n=14) and normotensive controls (n=14). Results: PE was associated with widespread differential methylation favoring hypermethylation. Pathway analysis identified the best matched process as a neuropeptide signaling pathway (p<10-5); best matched disease as eclampsia (p<9.97×10-20). Significantly differentially methylated genes (GRIN2b. GABRA1. PCDHB7, and BEX1) are associated with seizures. Conclusion: Altered maternal leukocyte DNA methylation is associated with PE at delivery, and differential methylation of certain neuronal genes may explain the risk for eclampsia.

AB - Objective: To compare genome-wide methylation profiles in maternal leukocyte DNA between normotensive and preeclamptic pregnant women at delivery. Methods: Age, body mass index matched case-control comparison of methylation at 27,578 cytosine- guanine sites in 14,495 genes in maternal leukocyte DNA in women with preeclampsia (PE; n=14) and normotensive controls (n=14). Results: PE was associated with widespread differential methylation favoring hypermethylation. Pathway analysis identified the best matched process as a neuropeptide signaling pathway (p<10-5); best matched disease as eclampsia (p<9.97×10-20). Significantly differentially methylated genes (GRIN2b. GABRA1. PCDHB7, and BEX1) are associated with seizures. Conclusion: Altered maternal leukocyte DNA methylation is associated with PE at delivery, and differential methylation of certain neuronal genes may explain the risk for eclampsia.

KW - DNA methylation

KW - Epigenetics

KW - Leukocyte

KW - Maternal

KW - Preeclampsia

KW - Pregnancy

UR - http://www.scopus.com/inward/record.url?scp=84881175477&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881175477&partnerID=8YFLogxK

U2 - 10.3109/10641955.2013.796970

DO - 10.3109/10641955.2013.796970

M3 - Article

C2 - 23782156

AN - SCOPUS:84881175477

VL - 32

SP - 257

EP - 269

JO - Hypertension in Pregnancy

JF - Hypertension in Pregnancy

SN - 1064-1955

IS - 3

ER -