Genome-wide gene expression profiling of cervical cancer in Hong Kong women by oligonucleotide microarray

Yick Fu Wong, Tak Hong Cheung, George S W Tsao, Keith W K Lo, So Fan Yim, Vivian W. Wang, Macy M S Heung, Samuel C S Chan, Loucia K Y Chan, Tina W F Ho, Katherine W Y Wong, Chen Li, Yu Guo, Tony K H Chung, David I Smith

Research output: Contribution to journalArticle

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Abstract

An analysis of gene expression profiles obtained from cervical cancers was performed to find those genes most aberrantly expressed. Total RNA was prepared from 29 samples of cervical squamous cell carcinoma and 18 control samples, and hybridized to Affymetrix oligonucleotide microarrays with probe sets complementary to over 20,000 transcripts. Unsupervised hierarchical clustering of the expression data readily distinguished normal cervix from cancer. Supervised analysis of gene expression data identified 98 and 139 genes that exhibited >2-fold upregulation and >2-fold down-regulation, respectively, in cervical cancer compared to normal cervix. Several of the genes that were differentially regulated included SPP1 (Osteopontin), CDKN2A (p16), RPL39L, Clorf1, MAL, p11, ARS and NICE-1, These were validated by quantitative RT-PCR on an independent set of cancer and control specimens. Gene Ontology analysis showed that the list of differentially expressed genes included ones that were involved in multiple biological processes, including cell proliferation, cell cycle and protein catabolism. Immunohistochemical staining of cancer specimens further confirmed differential expression of SPP1 in cervical cancer cells vs. nontumor cells. In addition, 2 genes, CTGF and RGS1 were found to be upregulated in late stage cancer compared to early stage cancer, suggesting that they might be involved in cancer progression. The pathway analysis of expression data showed that the SPP1, VEGF, CDC2 and CKS2 genes were coordinately differentially regulated between cancer and normal. The present study is promising and provides potential new insights into the extent of expression differences underlying the development and progression of cervical squamous cell cancer. This study has also revealed several genes that may be highly attractive candidate molecular markers/targets for cervical cancer diagnosis, prognosis and therapy.

Original languageEnglish (US)
Pages (from-to)2461-2469
Number of pages9
JournalInternational Journal of Cancer
Volume118
Issue number10
DOIs
StatePublished - May 15 2006

Fingerprint

Hong Kong
Gene Expression Profiling
Oligonucleotide Array Sequence Analysis
Uterine Cervical Neoplasms
Genome
Genes
Neoplasms
Biological Phenomena
Squamous Cell Neoplasms
Cell Cycle Proteins
Gene Ontology
Osteopontin
Transcriptome
Cervix Uteri
Vascular Endothelial Growth Factor A
Cluster Analysis
Squamous Cell Carcinoma
Up-Regulation
Down-Regulation
Cell Proliferation

Keywords

  • Cervical cancer
  • Gene expression
  • Microarray

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Genome-wide gene expression profiling of cervical cancer in Hong Kong women by oligonucleotide microarray. / Wong, Yick Fu; Cheung, Tak Hong; Tsao, George S W; Lo, Keith W K; Yim, So Fan; Wang, Vivian W.; Heung, Macy M S; Chan, Samuel C S; Chan, Loucia K Y; Ho, Tina W F; Wong, Katherine W Y; Li, Chen; Guo, Yu; Chung, Tony K H; Smith, David I.

In: International Journal of Cancer, Vol. 118, No. 10, 15.05.2006, p. 2461-2469.

Research output: Contribution to journalArticle

Wong, YF, Cheung, TH, Tsao, GSW, Lo, KWK, Yim, SF, Wang, VW, Heung, MMS, Chan, SCS, Chan, LKY, Ho, TWF, Wong, KWY, Li, C, Guo, Y, Chung, TKH & Smith, DI 2006, 'Genome-wide gene expression profiling of cervical cancer in Hong Kong women by oligonucleotide microarray', International Journal of Cancer, vol. 118, no. 10, pp. 2461-2469. https://doi.org/10.1002/ijc.21660
Wong, Yick Fu ; Cheung, Tak Hong ; Tsao, George S W ; Lo, Keith W K ; Yim, So Fan ; Wang, Vivian W. ; Heung, Macy M S ; Chan, Samuel C S ; Chan, Loucia K Y ; Ho, Tina W F ; Wong, Katherine W Y ; Li, Chen ; Guo, Yu ; Chung, Tony K H ; Smith, David I. / Genome-wide gene expression profiling of cervical cancer in Hong Kong women by oligonucleotide microarray. In: International Journal of Cancer. 2006 ; Vol. 118, No. 10. pp. 2461-2469.
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