Genome composition and tandemly repetitive sequence at some centromeres in the lizard Podarcis s. sicula Raf.

T. Capriglione, E. Olmo, G. Odierna, D. I. Smith, O. J. Miller

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

A detailed study on the genome of the lizard P. sicula has been carried out using restriction enzyme analysis followed by identification and cloning of a repetitive DNA fraction. The results show that P. sicula generally possesses a quite homogeneous genome composition, with a single tandemly repetitive sequence family that is easily visualized after digestion of genomic DNA with Taq I. The cloned repeating unit of this satellite (260 bp) has been designed pLCSl. In-situ hybridisation shows that this satellite is localized in the centromeric region. Dot blot experiments show that sequences similar to pLCSl are present in other species of the same family of lizards.

Original languageEnglish (US)
Pages (from-to)85-91
Number of pages7
JournalGenetica
Volume79
Issue number2
DOIs
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics
  • Plant Science
  • Insect Science

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