Genome composition and tandemly repetitive sequence at some centromeres in the lizard Podarcis s. sicula Raf.

T. Capriglione; E. Olmo; G. Odierna; D. I. Smith; O. J. Miller

doi:10.1007/BF00057925

Genome composition and tandemly repetitive sequence at some centromeres in the lizard Podarcis s. sicula Raf.

T. Capriglione, E. Olmo, G. Odierna, D. I. Smith, O. J. Miller

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

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Abstract

A detailed study on the genome of the lizard P. sicula has been carried out using restriction enzyme analysis followed by identification and cloning of a repetitive DNA fraction. The results show that P. sicula generally possesses a quite homogeneous genome composition, with a single tandemly repetitive sequence family that is easily visualized after digestion of genomic DNA with Taq I. The cloned repeating unit of this satellite (260 bp) has been designed pLCSl. In-situ hybridisation shows that this satellite is localized in the centromeric region. Dot blot experiments show that sequences similar to pLCSl are present in other species of the same family of lizards.

Original language	English (US)
Pages (from-to)	85-91
Number of pages	7
Journal	Genetica
Volume	79
Issue number	2
DOIs	https://doi.org/10.1007/BF00057925
State	Published - Jan 1989

ASJC Scopus subject areas

Animal Science and Zoology
Genetics
Plant Science
Insect Science

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10.1007/BF00057925

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abstract = "A detailed study on the genome of the lizard P. sicula has been carried out using restriction enzyme analysis followed by identification and cloning of a repetitive DNA fraction. The results show that P. sicula generally possesses a quite homogeneous genome composition, with a single tandemly repetitive sequence family that is easily visualized after digestion of genomic DNA with Taq I. The cloned repeating unit of this satellite (260 bp) has been designed pLCSl. In-situ hybridisation shows that this satellite is localized in the centromeric region. Dot blot experiments show that sequences similar to pLCSl are present in other species of the same family of lizards.",

author = "T. Capriglione and E. Olmo and G. Odierna and Smith, {D. I.} and Miller, {O. J.}",

year = "1989",

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AU - Capriglione, T.

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AU - Odierna, G.

AU - Smith, D. I.

AU - Miller, O. J.

PY - 1989/1

Y1 - 1989/1

N2 - A detailed study on the genome of the lizard P. sicula has been carried out using restriction enzyme analysis followed by identification and cloning of a repetitive DNA fraction. The results show that P. sicula generally possesses a quite homogeneous genome composition, with a single tandemly repetitive sequence family that is easily visualized after digestion of genomic DNA with Taq I. The cloned repeating unit of this satellite (260 bp) has been designed pLCSl. In-situ hybridisation shows that this satellite is localized in the centromeric region. Dot blot experiments show that sequences similar to pLCSl are present in other species of the same family of lizards.

AB - A detailed study on the genome of the lizard P. sicula has been carried out using restriction enzyme analysis followed by identification and cloning of a repetitive DNA fraction. The results show that P. sicula generally possesses a quite homogeneous genome composition, with a single tandemly repetitive sequence family that is easily visualized after digestion of genomic DNA with Taq I. The cloned repeating unit of this satellite (260 bp) has been designed pLCSl. In-situ hybridisation shows that this satellite is localized in the centromeric region. Dot blot experiments show that sequences similar to pLCSl are present in other species of the same family of lizards.

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Genome composition and tandemly repetitive sequence at some centromeres in the lizard Podarcis s. sicula Raf.

Abstract

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