Preliminary data suggesting high rates of diabetes, its risk factors, and complications among the Louisiana Coushatta Indians were the impetus for developing the Pennington Coushatta Diabetes Study Program in 1993. Studies performed to date have confirmed high rates of diabetes and obesity. If susceptability genes were identified, then development of better preventive measures for both conditions would be facilitated. Determining the degree of genetic variation in the population is a necessary first step towards identifying susceptabilty genes. Since October 1994, 95 individuals have been evaluated for diabetes and obesity. The mean ± S.E.M. age of those examined is 41.6 ± 2.7 years (range 9-83). Among the 95, 34 have confirmed diabetes and 11 have impaired glucose tolerance. For Coushatta members over the age of 18, the mean ± S.E.M. BMI is 29.0 kg/m2. A total of 21 microsatellite DNA markers spanning the autosomes have been genotyped on 21 individuals from 15 nuclear families, and three markers on the Y chromosome have been typed on the 12 males in this group of individuals. All of the autosomal markers show considerable variation in the population with at least three alleles being present at each locus. Heterozygosities range from 37% to 81%. One of the Y chromosome markers (DYS288) is identical in all the males tested, but three and four alleles are present for DYS389 and DYS390, respectively. Six different Y haplotypes are found among the 12 males. These results demonstrate that the variation that is essential for the success of linkage studies in identifying susceptability genes is present in the Coushatta population.
|Original language||English (US)|
|Journal||Journal of Investigative Medicine|
|State||Published - 1996|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)