Genetic risk factors for portopulmonary hypertension in patients with advanced liver disease

Kari E. Roberts, Michael B. Fallon, Michael J. Krowka, Robert S. Brown, James F. Trotter, Inga Peter, Hocine Tighiouart, James A. Knowles, Daniel Rabinowitz, Raymond L. Benza, David B. Badesch, Darren B. Taichman, Evelyn M. Horn, Steven Zacks, Neil Kaplowitz, Steven M. Kawut

Research output: Contribution to journalArticle

146 Scopus citations

Abstract

Rationale: Portopulmonary hypertension (PPHTN) occurs in 6% of liver transplant candidates. The pathogenesis of this complication of portal hypertension is poorly understood. Objectives: To identify genetic risk factors for PPHTN in patients with advanced liver disease. Methods: We performed a multicenter case-control study of patients with portal hypertension. Cases had a mean pulmonary artery pressure >25 mm Hg, pulmonary vascular resistance >240 dynes·s-1·cm-5, and pulmonary capillary wedge pressure ≤15 mm Hg. Controls had a right ventricular systolic pressure < 40 mm Hg (if estimated) and normal right-sided cardiac morphology by transthoracic echocardiography. We genotyped 1,079 common single nucleotide polymorphisms (SNPs) in 93 candidate genes in each patient. Measurements and Main Results: The study sample included 31 cases and 104 controls. Twenty-nine SNPs in 15 candidate genes were associated with the risk of PPHTN (P < 0.05). Multiple SNPs in the genes coding for estrogen receptor 1, aromatase, phosphodiesterase 5, angiopoietin 1, and calcium binding protein A4 were associated with the risk of PPHTN. The biological relevance of one of the aromatase SNPs was supported by an association with plasma estradiol levels. Conclusions: Genetic variation in estrogen signaling and cell growth regulators is associated with the risk of PPHTN. These biologic pathways may elucidate the mechanism for the development of PPHTN in certain patients with severe liver disease.

Original languageEnglish (US)
Pages (from-to)835-842
Number of pages8
JournalAmerican journal of respiratory and critical care medicine
Volume179
Issue number9
DOIs
StatePublished - May 1 2009

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Keywords

  • Genetic polymorphism
  • Hypertension
  • Portal hypertension
  • Pulmonary

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Roberts, K. E., Fallon, M. B., Krowka, M. J., Brown, R. S., Trotter, J. F., Peter, I., Tighiouart, H., Knowles, J. A., Rabinowitz, D., Benza, R. L., Badesch, D. B., Taichman, D. B., Horn, E. M., Zacks, S., Kaplowitz, N., & Kawut, S. M. (2009). Genetic risk factors for portopulmonary hypertension in patients with advanced liver disease. American journal of respiratory and critical care medicine, 179(9), 835-842. https://doi.org/10.1164/rccm.200809-1472OC