Genetic reconstitution of the high-affinity L-arabinose transport system

Bruce F Horazdovsky, R. W. Hogg

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Expression plasmids containing various portions of araFGH operon sequences were assayed for their ability to facilitate the high-affinity L-arabinose transport process in a strain lacking the chromosomal copy of this operon. Accumulation studies demonstrated that the specific induction of all three operon coding sequences was necessary to restore high-affinity L-arabinose transport. Kinetic analysis of this genetically reconstituted transport system indicated that it functions with essentially wild-type parameters. Therefore, L-arabinose-binding protein-mediated transport appears to require only two inducible membrane-associated components (araG and araH) in addition to the binding protein (araF).

Original languageEnglish (US)
Pages (from-to)3053-3059
Number of pages7
JournalJournal of Bacteriology
Volume171
Issue number6
StatePublished - 1989
Externally publishedYes

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Arabinose
Operon
Carrier Proteins
Protein Transport
Plasmids
Membranes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Horazdovsky, B. F., & Hogg, R. W. (1989). Genetic reconstitution of the high-affinity L-arabinose transport system. Journal of Bacteriology, 171(6), 3053-3059.

Genetic reconstitution of the high-affinity L-arabinose transport system. / Horazdovsky, Bruce F; Hogg, R. W.

In: Journal of Bacteriology, Vol. 171, No. 6, 1989, p. 3053-3059.

Research output: Contribution to journalArticle

Horazdovsky, BF & Hogg, RW 1989, 'Genetic reconstitution of the high-affinity L-arabinose transport system', Journal of Bacteriology, vol. 171, no. 6, pp. 3053-3059.
Horazdovsky, Bruce F ; Hogg, R. W. / Genetic reconstitution of the high-affinity L-arabinose transport system. In: Journal of Bacteriology. 1989 ; Vol. 171, No. 6. pp. 3053-3059.
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