Abstract
Expression plasmids containing various portions of araFGH operon sequences were assayed for their ability to facilitate the high-affinity L-arabinose transport process in a strain lacking the chromosomal copy of this operon. Accumulation studies demonstrated that the specific induction of all three operon coding sequences was necessary to restore high-affinity L-arabinose transport. Kinetic analysis of this genetically reconstituted transport system indicated that it functions with essentially wild-type parameters. Therefore, L-arabinose-binding protein-mediated transport appears to require only two inducible membrane-associated components (araG and araH) in addition to the binding protein (araF).
Original language | English (US) |
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Pages (from-to) | 3053-3059 |
Number of pages | 7 |
Journal | Journal of Bacteriology |
Volume | 171 |
Issue number | 6 |
DOIs | |
State | Published - 1989 |
ASJC Scopus subject areas
- Microbiology
- Molecular Biology