Genetic reconstitution of the high-affinity L-arabinose transport system

B. F. Horazdovsky, R. W. Hogg

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Expression plasmids containing various portions of araFGH operon sequences were assayed for their ability to facilitate the high-affinity L-arabinose transport process in a strain lacking the chromosomal copy of this operon. Accumulation studies demonstrated that the specific induction of all three operon coding sequences was necessary to restore high-affinity L-arabinose transport. Kinetic analysis of this genetically reconstituted transport system indicated that it functions with essentially wild-type parameters. Therefore, L-arabinose-binding protein-mediated transport appears to require only two inducible membrane-associated components (araG and araH) in addition to the binding protein (araF).

Original languageEnglish (US)
Pages (from-to)3053-3059
Number of pages7
JournalJournal of Bacteriology
Volume171
Issue number6
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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