Genetic features associated with 18 F-FDG uptake in intrahepatic cholangiocarcinoma

Keun Soo Ahn, Koo Jeong Kang, Yong Hoon Kim, Tae Seok Kim, Bong Il Song, Hae Won Kim, Daniel O’Brien, Lewis Rowland Roberts, Jeong Woo Lee, Kyoung Sook Won

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: In intrahepatic cholangiocarcinoma (iCCA), genetic characteristics on 18 F-fluorodeoxyglucose ( 18 F-FDG)-PET scans are not yet clarified. If specific genetic characteristics were found to be related to FDG uptake in iCCA, we can predict molecular features based on the FDG uptake patterns and to distinguish different types of treatments. In this purpose, we analyzed RNA sequencing in iCCA patients to evaluate gene expression signatures associated with FDG uptake patterns. Methods: We performed RNA sequencing of 22 cases iCCA who underwent preoperative 18 F-FDG-PET, and analyzed the clinical and molecular features according to the maximum standard uptake value (SUVmax). Genes and biological pathway which are associated with SUVmax were analyzed. Results: Patients with SUVmax higher than 9.0 (n = 9) had poorer disease-free survival than those with lower SUVmax (n = 13, P = 0.035). Genes related to glycolysis and gluconeogenesis, phosphorylation and cell cycle were significantly correlated with SUVmax (r ≥ 0.5). RRM2, which is related to the toxicity of Gemcitabine was positively correlated with SUVmax, and SLC27A2 which is associated with Cisplastin response was negatively correlated with SUVmax. According to the pathway analysis, cell cycle, cell division, hypoxia, inflammatory, and metabolism-related pathways were enriched in high SUVmax patients. Conclusion: The genomic features of gene expression and pathways can be predicted by FDG uptake features in iCCA. Patients with high FDG uptake have enriched cell cycle, metabolism and hypoxic pathways, which may lead to a more rational targeted treatment approach.

Original languageEnglish (US)
Pages (from-to)153-161
Number of pages9
JournalAnnals of Surgical Treatment and Research
Volume96
Issue number4
DOIs
StatePublished - Apr 1 2019

Fingerprint

Cholangiocarcinoma
RNA Sequence Analysis
Cell Cycle
Fluorodeoxyglucose F18
gemcitabine
Cell Hypoxia
Gluconeogenesis
Glycolysis
Transcriptome
Cell Division
Positron-Emission Tomography
Genes
Disease-Free Survival
Phosphorylation
Gene Expression
Therapeutics

Keywords

  • Cell cycle
  • Cholangiocarcinoma
  • Fluorodeoxyglucose F18
  • Gene expression
  • Positron-emission tomography

ASJC Scopus subject areas

  • Surgery

Cite this

Ahn, K. S., Kang, K. J., Kim, Y. H., Kim, T. S., Song, B. I., Kim, H. W., ... Won, K. S. (2019). Genetic features associated with 18 F-FDG uptake in intrahepatic cholangiocarcinoma Annals of Surgical Treatment and Research, 96(4), 153-161. https://doi.org/10.4174/astr.2019.96.4.153

Genetic features associated with 18 F-FDG uptake in intrahepatic cholangiocarcinoma . / Ahn, Keun Soo; Kang, Koo Jeong; Kim, Yong Hoon; Kim, Tae Seok; Song, Bong Il; Kim, Hae Won; O’Brien, Daniel; Roberts, Lewis Rowland; Lee, Jeong Woo; Won, Kyoung Sook.

In: Annals of Surgical Treatment and Research, Vol. 96, No. 4, 01.04.2019, p. 153-161.

Research output: Contribution to journalArticle

Ahn, KS, Kang, KJ, Kim, YH, Kim, TS, Song, BI, Kim, HW, O’Brien, D, Roberts, LR, Lee, JW & Won, KS 2019, ' Genetic features associated with 18 F-FDG uptake in intrahepatic cholangiocarcinoma ', Annals of Surgical Treatment and Research, vol. 96, no. 4, pp. 153-161. https://doi.org/10.4174/astr.2019.96.4.153
Ahn, Keun Soo ; Kang, Koo Jeong ; Kim, Yong Hoon ; Kim, Tae Seok ; Song, Bong Il ; Kim, Hae Won ; O’Brien, Daniel ; Roberts, Lewis Rowland ; Lee, Jeong Woo ; Won, Kyoung Sook. / Genetic features associated with 18 F-FDG uptake in intrahepatic cholangiocarcinoma In: Annals of Surgical Treatment and Research. 2019 ; Vol. 96, No. 4. pp. 153-161.
@article{33384a82dd7b4fb1bbf07517bb13d292,
title = "Genetic features associated with 18 F-FDG uptake in intrahepatic cholangiocarcinoma",
abstract = "Purpose: In intrahepatic cholangiocarcinoma (iCCA), genetic characteristics on 18 F-fluorodeoxyglucose ( 18 F-FDG)-PET scans are not yet clarified. If specific genetic characteristics were found to be related to FDG uptake in iCCA, we can predict molecular features based on the FDG uptake patterns and to distinguish different types of treatments. In this purpose, we analyzed RNA sequencing in iCCA patients to evaluate gene expression signatures associated with FDG uptake patterns. Methods: We performed RNA sequencing of 22 cases iCCA who underwent preoperative 18 F-FDG-PET, and analyzed the clinical and molecular features according to the maximum standard uptake value (SUVmax). Genes and biological pathway which are associated with SUVmax were analyzed. Results: Patients with SUVmax higher than 9.0 (n = 9) had poorer disease-free survival than those with lower SUVmax (n = 13, P = 0.035). Genes related to glycolysis and gluconeogenesis, phosphorylation and cell cycle were significantly correlated with SUVmax (r ≥ 0.5). RRM2, which is related to the toxicity of Gemcitabine was positively correlated with SUVmax, and SLC27A2 which is associated with Cisplastin response was negatively correlated with SUVmax. According to the pathway analysis, cell cycle, cell division, hypoxia, inflammatory, and metabolism-related pathways were enriched in high SUVmax patients. Conclusion: The genomic features of gene expression and pathways can be predicted by FDG uptake features in iCCA. Patients with high FDG uptake have enriched cell cycle, metabolism and hypoxic pathways, which may lead to a more rational targeted treatment approach.",
keywords = "Cell cycle, Cholangiocarcinoma, Fluorodeoxyglucose F18, Gene expression, Positron-emission tomography",
author = "Ahn, {Keun Soo} and Kang, {Koo Jeong} and Kim, {Yong Hoon} and Kim, {Tae Seok} and Song, {Bong Il} and Kim, {Hae Won} and Daniel O’Brien and Roberts, {Lewis Rowland} and Lee, {Jeong Woo} and Won, {Kyoung Sook}",
year = "2019",
month = "4",
day = "1",
doi = "10.4174/astr.2019.96.4.153",
language = "English (US)",
volume = "96",
pages = "153--161",
journal = "Annals of Surgical Treatment and Research",
issn = "2288-6575",
publisher = "Korean Surgical Society",
number = "4",

}

TY - JOUR

T1 - Genetic features associated with 18 F-FDG uptake in intrahepatic cholangiocarcinoma

AU - Ahn, Keun Soo

AU - Kang, Koo Jeong

AU - Kim, Yong Hoon

AU - Kim, Tae Seok

AU - Song, Bong Il

AU - Kim, Hae Won

AU - O’Brien, Daniel

AU - Roberts, Lewis Rowland

AU - Lee, Jeong Woo

AU - Won, Kyoung Sook

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Purpose: In intrahepatic cholangiocarcinoma (iCCA), genetic characteristics on 18 F-fluorodeoxyglucose ( 18 F-FDG)-PET scans are not yet clarified. If specific genetic characteristics were found to be related to FDG uptake in iCCA, we can predict molecular features based on the FDG uptake patterns and to distinguish different types of treatments. In this purpose, we analyzed RNA sequencing in iCCA patients to evaluate gene expression signatures associated with FDG uptake patterns. Methods: We performed RNA sequencing of 22 cases iCCA who underwent preoperative 18 F-FDG-PET, and analyzed the clinical and molecular features according to the maximum standard uptake value (SUVmax). Genes and biological pathway which are associated with SUVmax were analyzed. Results: Patients with SUVmax higher than 9.0 (n = 9) had poorer disease-free survival than those with lower SUVmax (n = 13, P = 0.035). Genes related to glycolysis and gluconeogenesis, phosphorylation and cell cycle were significantly correlated with SUVmax (r ≥ 0.5). RRM2, which is related to the toxicity of Gemcitabine was positively correlated with SUVmax, and SLC27A2 which is associated with Cisplastin response was negatively correlated with SUVmax. According to the pathway analysis, cell cycle, cell division, hypoxia, inflammatory, and metabolism-related pathways were enriched in high SUVmax patients. Conclusion: The genomic features of gene expression and pathways can be predicted by FDG uptake features in iCCA. Patients with high FDG uptake have enriched cell cycle, metabolism and hypoxic pathways, which may lead to a more rational targeted treatment approach.

AB - Purpose: In intrahepatic cholangiocarcinoma (iCCA), genetic characteristics on 18 F-fluorodeoxyglucose ( 18 F-FDG)-PET scans are not yet clarified. If specific genetic characteristics were found to be related to FDG uptake in iCCA, we can predict molecular features based on the FDG uptake patterns and to distinguish different types of treatments. In this purpose, we analyzed RNA sequencing in iCCA patients to evaluate gene expression signatures associated with FDG uptake patterns. Methods: We performed RNA sequencing of 22 cases iCCA who underwent preoperative 18 F-FDG-PET, and analyzed the clinical and molecular features according to the maximum standard uptake value (SUVmax). Genes and biological pathway which are associated with SUVmax were analyzed. Results: Patients with SUVmax higher than 9.0 (n = 9) had poorer disease-free survival than those with lower SUVmax (n = 13, P = 0.035). Genes related to glycolysis and gluconeogenesis, phosphorylation and cell cycle were significantly correlated with SUVmax (r ≥ 0.5). RRM2, which is related to the toxicity of Gemcitabine was positively correlated with SUVmax, and SLC27A2 which is associated with Cisplastin response was negatively correlated with SUVmax. According to the pathway analysis, cell cycle, cell division, hypoxia, inflammatory, and metabolism-related pathways were enriched in high SUVmax patients. Conclusion: The genomic features of gene expression and pathways can be predicted by FDG uptake features in iCCA. Patients with high FDG uptake have enriched cell cycle, metabolism and hypoxic pathways, which may lead to a more rational targeted treatment approach.

KW - Cell cycle

KW - Cholangiocarcinoma

KW - Fluorodeoxyglucose F18

KW - Gene expression

KW - Positron-emission tomography

UR - http://www.scopus.com/inward/record.url?scp=85063917935&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85063917935&partnerID=8YFLogxK

U2 - 10.4174/astr.2019.96.4.153

DO - 10.4174/astr.2019.96.4.153

M3 - Article

AN - SCOPUS:85063917935

VL - 96

SP - 153

EP - 161

JO - Annals of Surgical Treatment and Research

JF - Annals of Surgical Treatment and Research

SN - 2288-6575

IS - 4

ER -