Genetic expression and localization of nitric oxide synthase isoforms in normal and failing human myocardium obtained during cardiac transplantation

C. M. Wei, R. C. Daly, C. G A McGregor, J. S. McLaughlin, A. H. Foster

Research output: Contribution to journalArticle

Abstract

Nitric oxide (NO) is a potent paracrine factor that affects myocardial contractility and growth and that may be implicated in reperfusion injury, endothelial dysfunction, and allograft rejection. While both endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) isoforms have been detected in non-human mammalian tissues, the transcription, translation, and localization of both NOS isoforms in normal and failing human atrial and ventricular myocardium remains controversial. The present study was designed to investigate the genetic expression and localization of eNOS and iNOS in cardiac tissue obtained during cardiac transplantation in the presence (recipient) and absence (donor) of congestive heart failure (CHF). Atrial and ventricular tissue were obtained from 14 donors and 14 end-stage CHF patients undergoing cardiac transplantation. Northern blot analysis and in situ hybridization demonstrated similar levels of both eNOS and iNOS mRNA in atrial and ventricular tissue from normal and failing human hearts. Western blot analysis demonstrated that both eNOS and iNOS are present in human myocardium with no crossreactivity between eNOS and iNOS antibodies. Positive immunohistochemical staining was observed within the cytoplasm of atrial and ventricular cardiomyocytes. No significant differences in eNOS and iNOS immunoreactivity were detected. Both eNOS and iNOS activity was present at similar levels in normal and failing atrial and ventricular myocardium. In conclusion, the present studies demonstrate, for the first time, the integrated transcription, translation, and distribution of both eNOS and iNOS isoforms in normal and failing atrial and ventricular human myocardium.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997
Externally publishedYes

Fingerprint

heart transplant
Nitric Oxide Synthase Type III
Nitric Oxide Synthase Type II
Heart Transplantation
myocardium
nitric oxide synthase
Nitric Oxide Synthase
Myocardium
Protein Isoforms
Tissue
heart failure
Transcription
translation (genetics)
Heart Failure
transcription (genetics)
Tissue Donors
graft rejection
inducible nitric oxide synthase
endothelial nitric oxide synthase
Reperfusion Injury

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Genetic expression and localization of nitric oxide synthase isoforms in normal and failing human myocardium obtained during cardiac transplantation. / Wei, C. M.; Daly, R. C.; McGregor, C. G A; McLaughlin, J. S.; Foster, A. H.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

Wei, C. M. ; Daly, R. C. ; McGregor, C. G A ; McLaughlin, J. S. ; Foster, A. H. / Genetic expression and localization of nitric oxide synthase isoforms in normal and failing human myocardium obtained during cardiac transplantation. In: FASEB Journal. 1997 ; Vol. 11, No. 3.
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