TY - JOUR
T1 - Genetic diversity of hantaviruses isolated in China and characterization of novel hantaviruses isolated from Niviventer confucianus and Rattus rattus
AU - Wang, Hua
AU - Yoshimatsu, Kumiko
AU - Ebihara, Hideki
AU - Ogino, Michiko
AU - Araki, Koichi
AU - Kariwa, Hiroaki
AU - Wang, Zhaoxiao
AU - Luo, Zhaozhuang
AU - Li, Dexin
AU - Hang, Changshou
AU - Arikawa, Jiro
N1 - Funding Information:
We thank Hitoshi Suzuki, Graduate School of Environmental Earth Science, Hokkaido University, for helpful advice on the classification and evolution of rodents. This work was supported in part by a Grant-in-Aid for Scientific Research and for Development in Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan.
PY - 2000
Y1 - 2000
N2 - The antigenic and genetic properties of 46 hantaviruses from China, 13 from patients, 23 from rodents, and 10 from unknown hosts, were compared with those of other hantaviruses. The viruses were classified as either Hantaan (HTN) or Seoul (SEO) viruses. A phylogenetic analysis of the partial M (300 bp) and S (around 485 bp) genomes of HTN viruses identified nine distinct genetic subtypes, one consisting of isolates from Korea. The SEO viruses were divided into five genetic subtypes, although they had less variability than the HTN subtypes. There was a correlation between the subtype and province of origin for four subtypes of HTN viruses, confirming geographical clustering. Hantaan virus NC167 isolated from Niviventer confucianus and SEO virus Gou3 isolated from Rattus rattus were the basal clades in each virus. The phylogenetic trees constructed from the entire S and M segments suggested that NC167 was introduced to N. confucianus in a host-switching event. The reactivity of a panel of 35 monoclonal antibodies was almost exactly the same in NC167 and a representative HTN virus and in Gou3 and a representative SEO virus. However, there was a one-way cross-neutralization between them. These results confirm the varied nature of Murinae-associated hantaviruses in China.
AB - The antigenic and genetic properties of 46 hantaviruses from China, 13 from patients, 23 from rodents, and 10 from unknown hosts, were compared with those of other hantaviruses. The viruses were classified as either Hantaan (HTN) or Seoul (SEO) viruses. A phylogenetic analysis of the partial M (300 bp) and S (around 485 bp) genomes of HTN viruses identified nine distinct genetic subtypes, one consisting of isolates from Korea. The SEO viruses were divided into five genetic subtypes, although they had less variability than the HTN subtypes. There was a correlation between the subtype and province of origin for four subtypes of HTN viruses, confirming geographical clustering. Hantaan virus NC167 isolated from Niviventer confucianus and SEO virus Gou3 isolated from Rattus rattus were the basal clades in each virus. The phylogenetic trees constructed from the entire S and M segments suggested that NC167 was introduced to N. confucianus in a host-switching event. The reactivity of a panel of 35 monoclonal antibodies was almost exactly the same in NC167 and a representative HTN virus and in Gou3 and a representative SEO virus. However, there was a one-way cross-neutralization between them. These results confirm the varied nature of Murinae-associated hantaviruses in China.
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U2 - 10.1006/viro.2000.0630
DO - 10.1006/viro.2000.0630
M3 - Article
C2 - 11118357
AN - SCOPUS:0034534909
SN - 0042-6822
VL - 278
SP - 332
EP - 345
JO - Virology
JF - Virology
IS - 2
ER -