Genetic control of the immune response to haemoglobin. II. Studies using purified α-chain and β-chain as immunogens

C. J. Krco, A. L. Kazim, M. Z. Atassi, C. S. David

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Mice of independent haplotypes and several recombinant inbred strains were immunized with highly purified preparations of either the α-chain or β-chain subunit of human adult haemoglobin. Cells from the sensitized lymph nodes were challenged in vitro with the appropriate subunit (or in some cases both chains) and cell proliferation assessed by 3H-thymidine incorporation. Mice of the H-2(b) and H-2(d) haplotypes were high responders to α-chain while mice of the H-2(f), H-2(j), H-2(k), H-2(r), H-2(s), H-2(u), and H-2(v) haplotypes were low responders. The low responsiveness of B10.A(4R) and B10.MBR and the high responsiveness of B10 indicated that the Ir gene(s) determining responsiveness to the α-chain subunit resides in the I-A subregion of the mouse major histocompatibility complex. Mice of the H-2(d), H-2(f), and H-2(s) haplotypes were high responders and H-2(b), H-2(j), H-2(q), and H-2(u) haplotype mice were low responders to β-chain. H-2(k), H-2(p), H-2(r), and H-2(p) haplotype mice were intermediate responders to β-chain. The low responsiveness of B10.S(8R) and B10.TL and the high responsivenes of B10.S(9R) mapped to Ir gene(s) to β-chain to the I-A subregion. Data collected from challenging thigh responder cells with both subunits indicated that α-chain and β-chain do not crossreact. These results are discussed in reference to earlier observation suggesting that the low responsiveness of some strains of mice to priming and challenging using the intact haemoglobin molecule might be due to a negative regulatory influence mediated by one of the subunits. In the absence of this influence these mice would respond normally.

Original languageEnglish (US)
Pages (from-to)395-403
Number of pages9
JournalJournal of Immunogenetics
Volume8
Issue number5
StatePublished - 1981

Fingerprint

Hemoglobins
Haplotypes
Thigh
Major Histocompatibility Complex
Thymidine
Genes
Lymph Nodes
Cell Proliferation
Observation

ASJC Scopus subject areas

  • Genetics
  • Immunology

Cite this

Genetic control of the immune response to haemoglobin. II. Studies using purified α-chain and β-chain as immunogens. / Krco, C. J.; Kazim, A. L.; Atassi, M. Z.; David, C. S.

In: Journal of Immunogenetics, Vol. 8, No. 5, 1981, p. 395-403.

Research output: Contribution to journalArticle

Krco, C. J. ; Kazim, A. L. ; Atassi, M. Z. ; David, C. S. / Genetic control of the immune response to haemoglobin. II. Studies using purified α-chain and β-chain as immunogens. In: Journal of Immunogenetics. 1981 ; Vol. 8, No. 5. pp. 395-403.
@article{15512e085fb7469eb8ad6f24bba929ce,
title = "Genetic control of the immune response to haemoglobin. II. Studies using purified α-chain and β-chain as immunogens",
abstract = "Mice of independent haplotypes and several recombinant inbred strains were immunized with highly purified preparations of either the α-chain or β-chain subunit of human adult haemoglobin. Cells from the sensitized lymph nodes were challenged in vitro with the appropriate subunit (or in some cases both chains) and cell proliferation assessed by 3H-thymidine incorporation. Mice of the H-2(b) and H-2(d) haplotypes were high responders to α-chain while mice of the H-2(f), H-2(j), H-2(k), H-2(r), H-2(s), H-2(u), and H-2(v) haplotypes were low responders. The low responsiveness of B10.A(4R) and B10.MBR and the high responsiveness of B10 indicated that the Ir gene(s) determining responsiveness to the α-chain subunit resides in the I-A subregion of the mouse major histocompatibility complex. Mice of the H-2(d), H-2(f), and H-2(s) haplotypes were high responders and H-2(b), H-2(j), H-2(q), and H-2(u) haplotype mice were low responders to β-chain. H-2(k), H-2(p), H-2(r), and H-2(p) haplotype mice were intermediate responders to β-chain. The low responsiveness of B10.S(8R) and B10.TL and the high responsivenes of B10.S(9R) mapped to Ir gene(s) to β-chain to the I-A subregion. Data collected from challenging thigh responder cells with both subunits indicated that α-chain and β-chain do not crossreact. These results are discussed in reference to earlier observation suggesting that the low responsiveness of some strains of mice to priming and challenging using the intact haemoglobin molecule might be due to a negative regulatory influence mediated by one of the subunits. In the absence of this influence these mice would respond normally.",
author = "Krco, {C. J.} and Kazim, {A. L.} and Atassi, {M. Z.} and David, {C. S.}",
year = "1981",
language = "English (US)",
volume = "8",
pages = "395--403",
journal = "International Journal of Immunogenetics",
issn = "1744-3121",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Genetic control of the immune response to haemoglobin. II. Studies using purified α-chain and β-chain as immunogens

AU - Krco, C. J.

AU - Kazim, A. L.

AU - Atassi, M. Z.

AU - David, C. S.

PY - 1981

Y1 - 1981

N2 - Mice of independent haplotypes and several recombinant inbred strains were immunized with highly purified preparations of either the α-chain or β-chain subunit of human adult haemoglobin. Cells from the sensitized lymph nodes were challenged in vitro with the appropriate subunit (or in some cases both chains) and cell proliferation assessed by 3H-thymidine incorporation. Mice of the H-2(b) and H-2(d) haplotypes were high responders to α-chain while mice of the H-2(f), H-2(j), H-2(k), H-2(r), H-2(s), H-2(u), and H-2(v) haplotypes were low responders. The low responsiveness of B10.A(4R) and B10.MBR and the high responsiveness of B10 indicated that the Ir gene(s) determining responsiveness to the α-chain subunit resides in the I-A subregion of the mouse major histocompatibility complex. Mice of the H-2(d), H-2(f), and H-2(s) haplotypes were high responders and H-2(b), H-2(j), H-2(q), and H-2(u) haplotype mice were low responders to β-chain. H-2(k), H-2(p), H-2(r), and H-2(p) haplotype mice were intermediate responders to β-chain. The low responsiveness of B10.S(8R) and B10.TL and the high responsivenes of B10.S(9R) mapped to Ir gene(s) to β-chain to the I-A subregion. Data collected from challenging thigh responder cells with both subunits indicated that α-chain and β-chain do not crossreact. These results are discussed in reference to earlier observation suggesting that the low responsiveness of some strains of mice to priming and challenging using the intact haemoglobin molecule might be due to a negative regulatory influence mediated by one of the subunits. In the absence of this influence these mice would respond normally.

AB - Mice of independent haplotypes and several recombinant inbred strains were immunized with highly purified preparations of either the α-chain or β-chain subunit of human adult haemoglobin. Cells from the sensitized lymph nodes were challenged in vitro with the appropriate subunit (or in some cases both chains) and cell proliferation assessed by 3H-thymidine incorporation. Mice of the H-2(b) and H-2(d) haplotypes were high responders to α-chain while mice of the H-2(f), H-2(j), H-2(k), H-2(r), H-2(s), H-2(u), and H-2(v) haplotypes were low responders. The low responsiveness of B10.A(4R) and B10.MBR and the high responsiveness of B10 indicated that the Ir gene(s) determining responsiveness to the α-chain subunit resides in the I-A subregion of the mouse major histocompatibility complex. Mice of the H-2(d), H-2(f), and H-2(s) haplotypes were high responders and H-2(b), H-2(j), H-2(q), and H-2(u) haplotype mice were low responders to β-chain. H-2(k), H-2(p), H-2(r), and H-2(p) haplotype mice were intermediate responders to β-chain. The low responsiveness of B10.S(8R) and B10.TL and the high responsivenes of B10.S(9R) mapped to Ir gene(s) to β-chain to the I-A subregion. Data collected from challenging thigh responder cells with both subunits indicated that α-chain and β-chain do not crossreact. These results are discussed in reference to earlier observation suggesting that the low responsiveness of some strains of mice to priming and challenging using the intact haemoglobin molecule might be due to a negative regulatory influence mediated by one of the subunits. In the absence of this influence these mice would respond normally.

UR - http://www.scopus.com/inward/record.url?scp=0019785796&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019785796&partnerID=8YFLogxK

M3 - Article

C2 - 6795281

AN - SCOPUS:0019785796

VL - 8

SP - 395

EP - 403

JO - International Journal of Immunogenetics

JF - International Journal of Immunogenetics

SN - 1744-3121

IS - 5

ER -