Genetic Association of Bovine Lymphocyte Antigen DRB3 Alleles with Immunological Traits of Holstein Cattle

Allan B Dietz, J. C. Detilleux, A. E. Freeman, D. H. Kelley, J. R. Stabel, M. E. Kehrli

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

The associations between alleles at the BoLA (bovine lymphocyte antigen) DRB3 locus and 20 indicator traits of innate and adaptive immunity were investigated. Periparturient Holsteins (n = 127) were genotyped at the BoLA DRB3 locus using polymerase chain reaction and restriction fragment length polymorphism. Twenty-two alleles were observed in the study population, and frequencies ranged from 21 to <1%. The same cattle were tested for a total of 20 innate and adaptive immunity traits, including lymphocyte response to mitogens (proliferative responses and Ig secretion), serum Ig, complement and conglutinin concentrations, total leukocyte count, and selected assays for neutrophil function. Models with gene substitution effects were used to investigate associations between BoLA DRB3 alleles and each of the immunological variables. Significant associations were found with 13 of the leukocyte functions tested. The number of immune parameters with significant associations with any allele ranged from 0 (with alleles DRB3.2*23 and DRB3.2*27) to 7 (with DRB3.2*8). The immunological parameter that had the most associations with alleles was serum IgG2 concentration with 6 alleles. One group of 4 alleles (representing 46% of the total allele frequency) was uniformly associated with increased IgM and complement and decreased mononuclear cell numbers. Thus, we demonstrated that the BoLA DRB3 genotype can influence measures of innate and adaptive immunity.

Original languageEnglish (US)
Pages (from-to)400-405
Number of pages6
JournalJournal of Dairy Science
Volume80
Issue number2
StatePublished - Feb 1997

Fingerprint

lymphocyte antigens
Holstein
Alleles
Lymphocytes
alleles
Antigens
cattle
Adaptive Immunity
Innate Immunity
complement
loci
Serum
Leukocyte Count
leukocyte count
Mitogens
Gene Frequency
Restriction Fragment Length Polymorphisms
gene frequency
Immunoglobulin M
neutrophils

Keywords

  • Bovine lymphocyte antigen
  • Genetics
  • Major histocompatibility complex

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Science
  • veterinary(all)

Cite this

Dietz, A. B., Detilleux, J. C., Freeman, A. E., Kelley, D. H., Stabel, J. R., & Kehrli, M. E. (1997). Genetic Association of Bovine Lymphocyte Antigen DRB3 Alleles with Immunological Traits of Holstein Cattle. Journal of Dairy Science, 80(2), 400-405.

Genetic Association of Bovine Lymphocyte Antigen DRB3 Alleles with Immunological Traits of Holstein Cattle. / Dietz, Allan B; Detilleux, J. C.; Freeman, A. E.; Kelley, D. H.; Stabel, J. R.; Kehrli, M. E.

In: Journal of Dairy Science, Vol. 80, No. 2, 02.1997, p. 400-405.

Research output: Contribution to journalArticle

Dietz, AB, Detilleux, JC, Freeman, AE, Kelley, DH, Stabel, JR & Kehrli, ME 1997, 'Genetic Association of Bovine Lymphocyte Antigen DRB3 Alleles with Immunological Traits of Holstein Cattle', Journal of Dairy Science, vol. 80, no. 2, pp. 400-405.
Dietz, Allan B ; Detilleux, J. C. ; Freeman, A. E. ; Kelley, D. H. ; Stabel, J. R. ; Kehrli, M. E. / Genetic Association of Bovine Lymphocyte Antigen DRB3 Alleles with Immunological Traits of Holstein Cattle. In: Journal of Dairy Science. 1997 ; Vol. 80, No. 2. pp. 400-405.
@article{5960c1bfbfdf42679f5d90742a3b7dce,
title = "Genetic Association of Bovine Lymphocyte Antigen DRB3 Alleles with Immunological Traits of Holstein Cattle",
abstract = "The associations between alleles at the BoLA (bovine lymphocyte antigen) DRB3 locus and 20 indicator traits of innate and adaptive immunity were investigated. Periparturient Holsteins (n = 127) were genotyped at the BoLA DRB3 locus using polymerase chain reaction and restriction fragment length polymorphism. Twenty-two alleles were observed in the study population, and frequencies ranged from 21 to <1{\%}. The same cattle were tested for a total of 20 innate and adaptive immunity traits, including lymphocyte response to mitogens (proliferative responses and Ig secretion), serum Ig, complement and conglutinin concentrations, total leukocyte count, and selected assays for neutrophil function. Models with gene substitution effects were used to investigate associations between BoLA DRB3 alleles and each of the immunological variables. Significant associations were found with 13 of the leukocyte functions tested. The number of immune parameters with significant associations with any allele ranged from 0 (with alleles DRB3.2*23 and DRB3.2*27) to 7 (with DRB3.2*8). The immunological parameter that had the most associations with alleles was serum IgG2 concentration with 6 alleles. One group of 4 alleles (representing 46{\%} of the total allele frequency) was uniformly associated with increased IgM and complement and decreased mononuclear cell numbers. Thus, we demonstrated that the BoLA DRB3 genotype can influence measures of innate and adaptive immunity.",
keywords = "Bovine lymphocyte antigen, Genetics, Major histocompatibility complex",
author = "Dietz, {Allan B} and Detilleux, {J. C.} and Freeman, {A. E.} and Kelley, {D. H.} and Stabel, {J. R.} and Kehrli, {M. E.}",
year = "1997",
month = "2",
language = "English (US)",
volume = "80",
pages = "400--405",
journal = "Journal of Dairy Science",
issn = "0022-0302",
publisher = "Elsevier Limited",
number = "2",

}

TY - JOUR

T1 - Genetic Association of Bovine Lymphocyte Antigen DRB3 Alleles with Immunological Traits of Holstein Cattle

AU - Dietz, Allan B

AU - Detilleux, J. C.

AU - Freeman, A. E.

AU - Kelley, D. H.

AU - Stabel, J. R.

AU - Kehrli, M. E.

PY - 1997/2

Y1 - 1997/2

N2 - The associations between alleles at the BoLA (bovine lymphocyte antigen) DRB3 locus and 20 indicator traits of innate and adaptive immunity were investigated. Periparturient Holsteins (n = 127) were genotyped at the BoLA DRB3 locus using polymerase chain reaction and restriction fragment length polymorphism. Twenty-two alleles were observed in the study population, and frequencies ranged from 21 to <1%. The same cattle were tested for a total of 20 innate and adaptive immunity traits, including lymphocyte response to mitogens (proliferative responses and Ig secretion), serum Ig, complement and conglutinin concentrations, total leukocyte count, and selected assays for neutrophil function. Models with gene substitution effects were used to investigate associations between BoLA DRB3 alleles and each of the immunological variables. Significant associations were found with 13 of the leukocyte functions tested. The number of immune parameters with significant associations with any allele ranged from 0 (with alleles DRB3.2*23 and DRB3.2*27) to 7 (with DRB3.2*8). The immunological parameter that had the most associations with alleles was serum IgG2 concentration with 6 alleles. One group of 4 alleles (representing 46% of the total allele frequency) was uniformly associated with increased IgM and complement and decreased mononuclear cell numbers. Thus, we demonstrated that the BoLA DRB3 genotype can influence measures of innate and adaptive immunity.

AB - The associations between alleles at the BoLA (bovine lymphocyte antigen) DRB3 locus and 20 indicator traits of innate and adaptive immunity were investigated. Periparturient Holsteins (n = 127) were genotyped at the BoLA DRB3 locus using polymerase chain reaction and restriction fragment length polymorphism. Twenty-two alleles were observed in the study population, and frequencies ranged from 21 to <1%. The same cattle were tested for a total of 20 innate and adaptive immunity traits, including lymphocyte response to mitogens (proliferative responses and Ig secretion), serum Ig, complement and conglutinin concentrations, total leukocyte count, and selected assays for neutrophil function. Models with gene substitution effects were used to investigate associations between BoLA DRB3 alleles and each of the immunological variables. Significant associations were found with 13 of the leukocyte functions tested. The number of immune parameters with significant associations with any allele ranged from 0 (with alleles DRB3.2*23 and DRB3.2*27) to 7 (with DRB3.2*8). The immunological parameter that had the most associations with alleles was serum IgG2 concentration with 6 alleles. One group of 4 alleles (representing 46% of the total allele frequency) was uniformly associated with increased IgM and complement and decreased mononuclear cell numbers. Thus, we demonstrated that the BoLA DRB3 genotype can influence measures of innate and adaptive immunity.

KW - Bovine lymphocyte antigen

KW - Genetics

KW - Major histocompatibility complex

UR - http://www.scopus.com/inward/record.url?scp=0031067741&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031067741&partnerID=8YFLogxK

M3 - Article

C2 - 9058283

AN - SCOPUS:0031067741

VL - 80

SP - 400

EP - 405

JO - Journal of Dairy Science

JF - Journal of Dairy Science

SN - 0022-0302

IS - 2

ER -