Abstract
T cell activation is central to initiating an immune response. Two signals are required: an antigen-specific signal through the T cell receptor (TCR) and an antigen-independent costimulatory signal, primarily through CD28 in naïve T cells. Although many of the molecules involved in TCR signal transduction have been identified, the signaling pathways downstream of CD28 involved in costimulation are not well-defined. Through mutagenesis, we have generated a panel of Jurkat T cell lines in which CD28 costimulation fails to upregulate the RE/AP composite element of the IL-2 promoter. Biochemical analysis and genetic rescue of the defects in these cell lines will lead to a better understanding of CD28 signal transduction.
Original language | English (US) |
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Pages (from-to) | 513-520 |
Number of pages | 8 |
Journal | Immunologic Research |
Volume | 27 |
Issue number | 2-3 |
DOIs | |
State | Published - 2003 |
Keywords
- CD28
- RE/AP
- Signal transduction
ASJC Scopus subject areas
- Immunology