Generation of induced pluripotent stem cells

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Induced pluripotent stem cells (iPSCs) are generated from somatic cells that have been reprogrammed by the ectopic expression of defined embryonic transcription factors. This technology has provided investigators with a powerful tool for modeling disease and developing treatments for human disorders. This chapter will provide the researcher with some background on iPSCs and details on how to produce MEF-conditioned medium, prepare mitotically arrested mouse embryonic fibroblasts (MEFs), create iPSCs using viral vectors, passage iPSCs, and cryopreserve iPSCs. The methods offered here have been used in many laboratories around the world and the reader can initially follow these methods. However, not all cell types are easily transduced using viral vectors and other methods of delivering the reprogramming transcription factors may need to be tested.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages71-87
Number of pages17
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2221
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cryopreservation
  • Induced pluripotent stem cells
  • Mouse embryonic fibroblasts
  • Pluripotency
  • Reprogramming
  • iPSC isolation
  • iPSC passaging

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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