Generation of induced pluripotent stem cells

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Induced pluripotent stem cells (iPSCs) are generated from somatic cells that have been reprogrammed by the ectopic expression of defined embryonic transcription factors. This technology has provided investigators with a powerful tool for modelling disease and developing treatments for human disorders. This chapter provides the researcher with some background on iPSCs and details on how to produce MEFconditioned medium, prepare mitotically arrested mouse embryonic fibroblasts (MEFs), create iPSCs using viral vectors, passage iPSCs, and cryopreserve iPSCs. The methods offered here have been used in many laboratories around the world and the reader can initially follow these methods. However, not all cell types are easily transduced using viral vectors and other methods of delivering the reprogramming transcription factors may need to be tested.

Original languageEnglish (US)
Title of host publicationOsteoporosis and Osteoarthritis
PublisherSpringer New York
Pages43-58
Number of pages16
ISBN (Print)9781493916191, 9781493916184
DOIs
StatePublished - Oct 20 2014

Keywords

  • Cryopreservation
  • Induced pluripotent stem cells
  • iPSC isolation
  • iPSC passaging
  • Mouse embryonic fibroblasts
  • Pluripotency
  • Reprogramming

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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  • Cite this

    Deyle, D. R. (2014). Generation of induced pluripotent stem cells. In Osteoporosis and Osteoarthritis (pp. 43-58). Springer New York. https://doi.org/10.1007/978-1-4939-1619-1_5