Generation of cell hybrids via a fusogenic cell line

Siew Chiat Cheong, Isabelle Blangenois, Jean Denis Franssen, Charlotte Servais, Vy Phan, Myrto Trakatelli, Catherine Bruyns, Richard Vile, Thierry Velu, Annick Brandenburger

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Background: Hybrids obtained by fusion between tumour cells (TC) and dendritic cells (DC) have been proposed as anti-tumour vaccines because of their potential to combine the expression of tumour-associated antigens with efficient antigen presentation. The classical methods used for fusion, polyethylene glycol (PEG) and electrofusion, are cytotoxic and generate cell debris that can be taken up by DC rendering the identification of true hybrids difficult. Methods: We have established a stable cell line expressing a viral fusogenic membrane glycoprotein (FMG) that is not itself susceptible to fusion. This cell line has been used to generate hybrids and to evaluate the relevance of tools used for hybrid detection. Results: This FMG-expressing cell line promotes fusion between autologous or allogeneic TC and DC in any combination, generating 'tri-parental hybrids'. At least 20% of TC are found to be integrated into hybrids. Conclusions: It is speculated that this tri-parental hybrid approach offers new possibilities to further modulate the anti-tumour effect of the DC/TC hybrids since it allows the expression of relevant immunostimulatory molecules by appropriate engineering of the fusogenic cell line.

Original languageEnglish (US)
Pages (from-to)919-928
Number of pages10
JournalJournal of Gene Medicine
Volume8
Issue number7
DOIs
StatePublished - Jul 1 2006

Keywords

  • Dendritic cell-tumour cell hybrids
  • Immunotherapy
  • Viral fusogenic membrane glycoprotein

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Drug Discovery
  • Genetics(clinical)

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