TY - JOUR
T1 - Generation of cell hybrids via a fusogenic cell line
AU - Cheong, Siew Chiat
AU - Blangenois, Isabelle
AU - Franssen, Jean Denis
AU - Servais, Charlotte
AU - Phan, Vy
AU - Trakatelli, Myrto
AU - Bruyns, Catherine
AU - Vile, Richard
AU - Velu, Thierry
AU - Brandenburger, Annick
PY - 2006/7
Y1 - 2006/7
N2 - Background: Hybrids obtained by fusion between tumour cells (TC) and dendritic cells (DC) have been proposed as anti-tumour vaccines because of their potential to combine the expression of tumour-associated antigens with efficient antigen presentation. The classical methods used for fusion, polyethylene glycol (PEG) and electrofusion, are cytotoxic and generate cell debris that can be taken up by DC rendering the identification of true hybrids difficult. Methods: We have established a stable cell line expressing a viral fusogenic membrane glycoprotein (FMG) that is not itself susceptible to fusion. This cell line has been used to generate hybrids and to evaluate the relevance of tools used for hybrid detection. Results: This FMG-expressing cell line promotes fusion between autologous or allogeneic TC and DC in any combination, generating 'tri-parental hybrids'. At least 20% of TC are found to be integrated into hybrids. Conclusions: It is speculated that this tri-parental hybrid approach offers new possibilities to further modulate the anti-tumour effect of the DC/TC hybrids since it allows the expression of relevant immunostimulatory molecules by appropriate engineering of the fusogenic cell line.
AB - Background: Hybrids obtained by fusion between tumour cells (TC) and dendritic cells (DC) have been proposed as anti-tumour vaccines because of their potential to combine the expression of tumour-associated antigens with efficient antigen presentation. The classical methods used for fusion, polyethylene glycol (PEG) and electrofusion, are cytotoxic and generate cell debris that can be taken up by DC rendering the identification of true hybrids difficult. Methods: We have established a stable cell line expressing a viral fusogenic membrane glycoprotein (FMG) that is not itself susceptible to fusion. This cell line has been used to generate hybrids and to evaluate the relevance of tools used for hybrid detection. Results: This FMG-expressing cell line promotes fusion between autologous or allogeneic TC and DC in any combination, generating 'tri-parental hybrids'. At least 20% of TC are found to be integrated into hybrids. Conclusions: It is speculated that this tri-parental hybrid approach offers new possibilities to further modulate the anti-tumour effect of the DC/TC hybrids since it allows the expression of relevant immunostimulatory molecules by appropriate engineering of the fusogenic cell line.
KW - Dendritic cell-tumour cell hybrids
KW - Immunotherapy
KW - Viral fusogenic membrane glycoprotein
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U2 - 10.1002/jgm.906
DO - 10.1002/jgm.906
M3 - Article
C2 - 16602137
AN - SCOPUS:33746217551
SN - 1099-498X
VL - 8
SP - 919
EP - 928
JO - Journal of Gene Medicine
JF - Journal of Gene Medicine
IS - 7
ER -