Generation and selection of a panel of pan-filovirus single-chain antibodies using cell-free ribosome display

Adinarayana Kunamneni, Elizabeth C. Clarke, Chunyan Ye, Steven B. Bradfute, Ravi Durvasula

Research output: Contribution to journalArticlepeer-review

Abstract

Filoviruses, which include ebolaviruses and marburgvirus, can cause outbreaks of highly lethal hemorrhagic fever. This disease causes significant morbidity and mortality in humans and non-human primates, with human fatality rates reaching 90% during some outbreaks. Currently, there is lack of licensed vaccines or antivirals for these viruses. Since early symptoms of filovirus infection mimic more common diseases, there is a strong unmet public health and biodefense need for broad-spectrum filovirus rapid diagnostics. We have generated a panel of mouse single-chain Fvantibodies (scFvs) to filovirus glycoproteins (GPs) using cell-free ribosome display and determined their cross-reactivity profiles to all known filovirus species. Two scFvs (4-2 and 22-1) were able to detect all known Ebolavirus and Marburgvirus species. This is the first report on ribosome display scFvs that can detect a broad set of filovirus GPs, which demonstrates the potential for use in diagnostics.

Original languageEnglish (US)
Pages (from-to)198-206
Number of pages9
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume101
Issue number1
DOIs
StatePublished - 2019

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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