Generation and characterization of human induced pluripotent stem cell (hiPSC) lines from an Alzheimer's disease (ASUi001-A) and non-demented control (ASUi002-A) patient homozygous for the Apolipoprotein e4 (APOE4) risk variant

Nicholas Brookhouser, Ping Zhang, Richard John Caselli, Jean J. Kim, David A. Brafman

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Although the majority of late-onset Alzheimer's disease (AD) patients are labeled sporadic, multiple genetic risk variants have been identified, the most powerful and prevalent of which is the e4 variant of the Apolipoprotein E (APOE) gene. Here, we generated human induced pluripotent stem cell (hiPSC) lines from the peripheral blood mononuclear cells (PBMCs) of a clinically diagnosed AD patient [ASUi001-A] and a non-demented control (NDC) patient [ASUi002-A] homozygous for the APOE4 risk allele. These hiPSCs maintained their original genotype, expressed pluripotency markers, exhibited a normal karyotype, and demonstrated the ability to differentiate into cells representative of the three germ layers.

Original languageEnglish (US)
JournalStem Cell Research
DOIs
StateAccepted/In press - Feb 28 2017

Fingerprint

Apolipoprotein E4
Induced Pluripotent Stem Cells
Alzheimer Disease
Cell Line
Germ Layers
Aptitude
Apolipoproteins E
Karyotype
Blood Cells
Alleles
Genotype
Genes

ASJC Scopus subject areas

  • Medicine(all)
  • Developmental Biology
  • Cell Biology

Cite this

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abstract = "Although the majority of late-onset Alzheimer's disease (AD) patients are labeled sporadic, multiple genetic risk variants have been identified, the most powerful and prevalent of which is the e4 variant of the Apolipoprotein E (APOE) gene. Here, we generated human induced pluripotent stem cell (hiPSC) lines from the peripheral blood mononuclear cells (PBMCs) of a clinically diagnosed AD patient [ASUi001-A] and a non-demented control (NDC) patient [ASUi002-A] homozygous for the APOE4 risk allele. These hiPSCs maintained their original genotype, expressed pluripotency markers, exhibited a normal karyotype, and demonstrated the ability to differentiate into cells representative of the three germ layers.",
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AU - Brookhouser, Nicholas

AU - Zhang, Ping

AU - Caselli, Richard John

AU - Kim, Jean J.

AU - Brafman, David A.

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