General and cell-type specific mechanisms target TRPP2/PKD-2 to cilia

Young Kyung Bae, Hongmin Qin, Karla M. Knobel, Jinghua Hu, Joel L. Rosenbaum, Maureen M. Barr

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Ciliary localization of the transient receptor potential polycystin 2 channel (TRPP2/PKD-2) is evolutionarily conserved, but how TRPP2 is targeted to cilia is not known. In this study, we characterize the motility and localization of PKD-2, a TRPP2 homolog, in C. elegans sensory neurons. We demonstrate that GFP-tagged PKD-2 moves bidirectionally in the dendritic compartment. Furthermore, we show a requirement for different molecules in regulating the ciliary localization of PKD-2. PKD-2 is directed to moving dendritic particles by the UNC-101/adaptor protein 1 (AP-1) complex. When expressed in non-native neurons, PKD-2 remains in cell bodies and is not observed in dendrites or cilia, indicating that cell-type specific factors are required for directing PKD-2 to the dendrite. PKD-2 stabilization in cilia and cell bodies requires LOV-1, a functional partner and a TRPP1 homolog. In lov-1 mutants, PKD-2 is greatly reduced in cilia and forms abnormal aggregates in neuronal cell bodies. Intraflagellar transport (IFT) is not essential for PKD-2 dendritic motility or access to the cilium, but may regulate PKD-2 ciliary abundance. We propose that both general and cell-type-specific factors govern TRPP2/PKD-2 subcellular distribution by forming at least two steps involving somatodendritic and ciliary sorting decisions.

Original languageEnglish (US)
Pages (from-to)3859-3870
Number of pages12
JournalDevelopment
Volume133
Issue number19
DOIs
StatePublished - Oct 2006
Externally publishedYes

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Cilia
Dendrites
Adaptor Protein Complex 1
Sensory Receptor Cells
Neurons
Cell Body

Keywords

  • Autosomal dominant polycystic kidney disease
  • C. elegans
  • TRPP2 (PKD2)/PKD-2

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Bae, Y. K., Qin, H., Knobel, K. M., Hu, J., Rosenbaum, J. L., & Barr, M. M. (2006). General and cell-type specific mechanisms target TRPP2/PKD-2 to cilia. Development, 133(19), 3859-3870. https://doi.org/10.1242/dev.02555

General and cell-type specific mechanisms target TRPP2/PKD-2 to cilia. / Bae, Young Kyung; Qin, Hongmin; Knobel, Karla M.; Hu, Jinghua; Rosenbaum, Joel L.; Barr, Maureen M.

In: Development, Vol. 133, No. 19, 10.2006, p. 3859-3870.

Research output: Contribution to journalArticle

Bae, YK, Qin, H, Knobel, KM, Hu, J, Rosenbaum, JL & Barr, MM 2006, 'General and cell-type specific mechanisms target TRPP2/PKD-2 to cilia', Development, vol. 133, no. 19, pp. 3859-3870. https://doi.org/10.1242/dev.02555
Bae YK, Qin H, Knobel KM, Hu J, Rosenbaum JL, Barr MM. General and cell-type specific mechanisms target TRPP2/PKD-2 to cilia. Development. 2006 Oct;133(19):3859-3870. https://doi.org/10.1242/dev.02555
Bae, Young Kyung ; Qin, Hongmin ; Knobel, Karla M. ; Hu, Jinghua ; Rosenbaum, Joel L. ; Barr, Maureen M. / General and cell-type specific mechanisms target TRPP2/PKD-2 to cilia. In: Development. 2006 ; Vol. 133, No. 19. pp. 3859-3870.
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