TY - JOUR
T1 - Gene transcription abnormalities in canine atopic dermatitis and related human eosinophilic allergic diseases
AU - Plager, Douglas A.
AU - Torres, Sheila M.F.
AU - Koch, Sandra N.
AU - Kita, Hirohito
PY - 2012/9/15
Y1 - 2012/9/15
N2 - Canine atopic dermatitis (AD) is clinically similar to human AD, implicating it as a useful model of human eosinophilic allergic disease. To identify cutaneous gene transcription changes in relatively early inflammation of canine AD, microarrays were used to monitor transcription in normal skin (n= 13) and in acute lesional AD (ALAD) and nearby visibly nonlesional AD (NLAD) skin (n= 13) from dogs. Scanning the putative abnormally transcribed genes, several potentially relevant genes, some abnormally transcribed in both NLAD and ALAD (e.g. IL6, NFAM1, MSRA, and SYK), were observed. Comparison for abnormally transcribed genes common to two related human diseases, human AD and asthmatic chronic rhinosinusitis with nasal polyps (aCRSwNP), further identified genes or gene sets likely relevant to eosinophilic allergic inflammation. These included: (1) genes associated with alternatively activated monocyte-derived cells, including members of the monocyte chemotactic protein (MCP) gene cluster, (2) members of the IL1 family gene cluster, (3) eosinophil-associated seven transmembrane receptor EMR1 and EMR3 genes, (4) interferon-inducible genes, and (5) keratin genes associated with hair and nail formation. Overall, numerous abnormally transcribed genes were observed only in canine AD; however, many others are common to related human eosinophilic allergic diseases and represent therapeutic targets testable in dogs with AD.
AB - Canine atopic dermatitis (AD) is clinically similar to human AD, implicating it as a useful model of human eosinophilic allergic disease. To identify cutaneous gene transcription changes in relatively early inflammation of canine AD, microarrays were used to monitor transcription in normal skin (n= 13) and in acute lesional AD (ALAD) and nearby visibly nonlesional AD (NLAD) skin (n= 13) from dogs. Scanning the putative abnormally transcribed genes, several potentially relevant genes, some abnormally transcribed in both NLAD and ALAD (e.g. IL6, NFAM1, MSRA, and SYK), were observed. Comparison for abnormally transcribed genes common to two related human diseases, human AD and asthmatic chronic rhinosinusitis with nasal polyps (aCRSwNP), further identified genes or gene sets likely relevant to eosinophilic allergic inflammation. These included: (1) genes associated with alternatively activated monocyte-derived cells, including members of the monocyte chemotactic protein (MCP) gene cluster, (2) members of the IL1 family gene cluster, (3) eosinophil-associated seven transmembrane receptor EMR1 and EMR3 genes, (4) interferon-inducible genes, and (5) keratin genes associated with hair and nail formation. Overall, numerous abnormally transcribed genes were observed only in canine AD; however, many others are common to related human eosinophilic allergic diseases and represent therapeutic targets testable in dogs with AD.
KW - Allergy
KW - Asthma
KW - Atopic dermatitis
KW - Canine
KW - Dog
KW - Eczema
KW - Eosinophil
KW - Gene expression
KW - High-density oligonucleotide arrays
KW - Microarray
KW - Rhinitis
KW - Sinusitis
UR - http://www.scopus.com/inward/record.url?scp=84865564100&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84865564100&partnerID=8YFLogxK
U2 - 10.1016/j.vetimm.2012.06.003
DO - 10.1016/j.vetimm.2012.06.003
M3 - Article
C2 - 22749291
AN - SCOPUS:84865564100
SN - 0165-2427
VL - 149
SP - 136
EP - 142
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
IS - 1-2
ER -