Gene signatures related to HAI response following influenza A/H1N1 vaccine in older individuals

Inna G. Ovsyannikova, Ann L Oberg, Richard B Kennedy, Michael T. Zimmermann, Iana H. Haralambieva, Krista M. Goergen, Diane E. Grill, Gregory A. Poland

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

To assess gene signatures related to humoral response among healthy older subjects following seasonal influenza vaccination, we studied 94 healthy adults (50–74 years old) who received one documented dose of licensed trivalent influenza vaccine containing the A/California/7/2009 (H1N1)-like virus strain. Influenza-specific antibody (HAI) titer in serum samples and next-generation sequencing on PBMCs were performed using blood samples collected prior to (Day 0) and at two timepoints after (Days 3 and 28) vaccination. We identified a number of uncharacterized genes (ZNF300, NUP1333, KLK1 and others) and confirmed previous studies demonstrating specific genes/genesets that are important mediators of host immune responses and that displayed associations with antibody response to influenza A/H1N1 vaccine. These included interferon-regulatory transcription factors (IRF1/IRF2/IRF6/IRF7/IRF9), chemokine/chemokine receptors (CCR5/CCR9/CCL5), cytokine/cytokine receptors (IFNG/IL10RA/TNFRSF1A), protein kinases (MAP2K4/MAPK3), growth factor receptor (TGFBR1). The identification of gene signatures associated with antibody response represents an early stage in the science for which further research is needed. Such research may assist in the design of better vaccines to facilitate improved defenses against new influenza virus strains, as well as better understanding the genetic drivers of immune responses.

Original languageEnglish (US)
Article numbere00098
JournalHeliyon
Volume2
Issue number5
DOIs
StatePublished - May 1 2016

Fingerprint

Human Influenza
Vaccines
Genes
Antibody Formation
Vaccination
Interferon Regulatory Factors
H1N1 Subtype Influenza A Virus
Cytokine Receptors
Growth Factor Receptors
Influenza Vaccines
Chemokine Receptors
Orthomyxoviridae
Chemokines
Research
Protein Kinases
Healthy Volunteers
Transcription Factors
Cytokines
Antibodies
Serum

Keywords

  • Genetics
  • Immunology

ASJC Scopus subject areas

  • General

Cite this

Ovsyannikova, I. G., Oberg, A. L., Kennedy, R. B., Zimmermann, M. T., Haralambieva, I. H., Goergen, K. M., ... Poland, G. A. (2016). Gene signatures related to HAI response following influenza A/H1N1 vaccine in older individuals. Heliyon, 2(5), [e00098]. https://doi.org/10.1016/j.heliyon.2016.e00098

Gene signatures related to HAI response following influenza A/H1N1 vaccine in older individuals. / Ovsyannikova, Inna G.; Oberg, Ann L; Kennedy, Richard B; Zimmermann, Michael T.; Haralambieva, Iana H.; Goergen, Krista M.; Grill, Diane E.; Poland, Gregory A.

In: Heliyon, Vol. 2, No. 5, e00098, 01.05.2016.

Research output: Contribution to journalArticle

Ovsyannikova, IG, Oberg, AL, Kennedy, RB, Zimmermann, MT, Haralambieva, IH, Goergen, KM, Grill, DE & Poland, GA 2016, 'Gene signatures related to HAI response following influenza A/H1N1 vaccine in older individuals', Heliyon, vol. 2, no. 5, e00098. https://doi.org/10.1016/j.heliyon.2016.e00098
Ovsyannikova, Inna G. ; Oberg, Ann L ; Kennedy, Richard B ; Zimmermann, Michael T. ; Haralambieva, Iana H. ; Goergen, Krista M. ; Grill, Diane E. ; Poland, Gregory A. / Gene signatures related to HAI response following influenza A/H1N1 vaccine in older individuals. In: Heliyon. 2016 ; Vol. 2, No. 5.
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