Gene profiling of cell cycle progression through S-phase reveals sequential expression of genes required for DNA replication and nucleosome assembly

Caroline M.J. Van der Meijden, Mai X. Luong, Daniel Peric-Hupkes, Brian Cho, Janet L. Stein, Andre J. Van Wijnen, Gary S. Stein, David S. Lapointe

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

The ordered expression of genes after growth factor stimulation in G1 supports the onset of DNA replication. To characterize regulatory events during S-phase when cell cycle progression has become growth factor independent, we have profiled the expression of over 7,000 human genes using GeneChip DNA microarray analysis. HeLa cells were synchronized at the beginning of S-phase by thymidine/aphidicolin block, and RNA populations were analyzed throughout the S and G2 phases. Expression of genes involved in DNA replication is maximal during early S-phase, whereas histone mRNAs peak at mid S-phase. Genes related to cell proliferation, including those encoding cyclins, oncoproteins, growth factors, proteins involved in signal transduction, and DNA repair proteins, follow distinct temporal patterns of expression that are functionally linked to initiation of DNA replication and progression through S-phase. The timing of expression for many genes in tumor-derived HeLa cells is highly conserved when compared with normal cells. In contrast, a number of genes show growth phenotype-related expression patterns that may directly reflect loss of stringent growth control in tumor cells. Our data reveal there is a core subset of cell growth-related genes that is fundamental to cycling cells irrespective of cell growth phenotype.

Original languageEnglish (US)
Pages (from-to)3233-3243
Number of pages11
JournalCancer research
Volume62
Issue number11
StatePublished - Jun 1 2002

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Van der Meijden, C. M. J., Luong, M. X., Peric-Hupkes, D., Cho, B., Stein, J. L., Van Wijnen, A. J., Stein, G. S., & Lapointe, D. S. (2002). Gene profiling of cell cycle progression through S-phase reveals sequential expression of genes required for DNA replication and nucleosome assembly. Cancer research, 62(11), 3233-3243.