Gene expression profiling of tuberculous meningitis co-infected with HIV

Ghantasala S. Sameer Kumar, Abhilash K. Venuaopal, Manoi Kumar Kashvap, Raiesh Raiu, Arivusudar Marimuthu, Shvam Mohan Palapetta, Yashwanth Subbanayya, Renu Goel, Ankit Chawla, Jyoti Bajpai Dikshit, Pramila Tata, H. C. Harsha, Jagadeesha Maharudraiah, Y. L. Ramachandra, Parthasarathy Satishchandra, T. S. Keshava Prasad, Akhilesh Pandey, Anita Mahadevan, S. K. Shankar

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Tuberculous meningitis (TBM) is a fatal form of Mycobacterium tuberculosis infection of the central nervous system (CNS). The similarities in the clinical and radiological findings in TBM cases with or without HIV make the diagnosis very challenging. Identification of genes, which are differentially expressed in brain tissues of HIV positive and HIV negative TBM patients, would enable better understanding of the molecular aspects of the infection and would also serve as an initial platform to evaluate potential biomarkers. Here, we report the identification of 796 differentially regulated genes in brain tissues of TBM patients co-infected with HIV using oligonucleotide DNA microarrays. We also performed immunohistochemical validation and confirmed the abundance of four gene products-glial fibrillary acidic protein (GFAP), serpin peptidase inhibitor, clade A member 3 (SERPINA3), thymidine phosphorylase (TYMP/ ECGF1) and heat shock 70 kDa protein 8 (HSPA8). Our study paves the way for understanding the mechanism of BM in HIV positive patients and for further validation of potential disease biomarkers.

Original languageEnglish (US)
Pages (from-to)235-244
Number of pages10
JournalJournal of Proteomics and Bioinformatics
Volume5
Issue number9
DOIs
StatePublished - 2012

Keywords

  • Cerebrospinal fluid
  • Early diagnosis
  • GeneSpring
  • Neurological disorder
  • Single color labeling

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Computer Science Applications
  • Cell Biology

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