TY - JOUR
T1 - Gene expression profiling of tuberculous meningitis co-infected with HIV
AU - Sameer Kumar, Ghantasala S.
AU - Venuaopal, Abhilash K.
AU - Kashvap, Manoi Kumar
AU - Raiu, Raiesh
AU - Marimuthu, Arivusudar
AU - Palapetta, Shvam Mohan
AU - Subbanayya, Yashwanth
AU - Goel, Renu
AU - Chawla, Ankit
AU - Dikshit, Jyoti Bajpai
AU - Tata, Pramila
AU - Harsha, H. C.
AU - Maharudraiah, Jagadeesha
AU - Ramachandra, Y. L.
AU - Satishchandra, Parthasarathy
AU - Keshava Prasad, T. S.
AU - Pandey, Akhilesh
AU - Mahadevan, Anita
AU - Shankar, S. K.
PY - 2012
Y1 - 2012
N2 - Tuberculous meningitis (TBM) is a fatal form of Mycobacterium tuberculosis infection of the central nervous system (CNS). The similarities in the clinical and radiological findings in TBM cases with or without HIV make the diagnosis very challenging. Identification of genes, which are differentially expressed in brain tissues of HIV positive and HIV negative TBM patients, would enable better understanding of the molecular aspects of the infection and would also serve as an initial platform to evaluate potential biomarkers. Here, we report the identification of 796 differentially regulated genes in brain tissues of TBM patients co-infected with HIV using oligonucleotide DNA microarrays. We also performed immunohistochemical validation and confirmed the abundance of four gene products-glial fibrillary acidic protein (GFAP), serpin peptidase inhibitor, clade A member 3 (SERPINA3), thymidine phosphorylase (TYMP/ ECGF1) and heat shock 70 kDa protein 8 (HSPA8). Our study paves the way for understanding the mechanism of BM in HIV positive patients and for further validation of potential disease biomarkers.
AB - Tuberculous meningitis (TBM) is a fatal form of Mycobacterium tuberculosis infection of the central nervous system (CNS). The similarities in the clinical and radiological findings in TBM cases with or without HIV make the diagnosis very challenging. Identification of genes, which are differentially expressed in brain tissues of HIV positive and HIV negative TBM patients, would enable better understanding of the molecular aspects of the infection and would also serve as an initial platform to evaluate potential biomarkers. Here, we report the identification of 796 differentially regulated genes in brain tissues of TBM patients co-infected with HIV using oligonucleotide DNA microarrays. We also performed immunohistochemical validation and confirmed the abundance of four gene products-glial fibrillary acidic protein (GFAP), serpin peptidase inhibitor, clade A member 3 (SERPINA3), thymidine phosphorylase (TYMP/ ECGF1) and heat shock 70 kDa protein 8 (HSPA8). Our study paves the way for understanding the mechanism of BM in HIV positive patients and for further validation of potential disease biomarkers.
KW - Cerebrospinal fluid
KW - Early diagnosis
KW - GeneSpring
KW - Neurological disorder
KW - Single color labeling
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U2 - 10.4172/Jpb.1000243
DO - 10.4172/Jpb.1000243
M3 - Article
AN - SCOPUS:84867667525
SN - 0974-276X
VL - 5
SP - 235
EP - 244
JO - Journal of Proteomics and Bioinformatics
JF - Journal of Proteomics and Bioinformatics
IS - 9
ER -