TY - JOUR
T1 - Gene Expression Profiling of Gastric Cancer
AU - Marimuthu, Arivusudar
AU - Jacob, Harrys K.C.
AU - Jakharia, Aniruddha
AU - Subbannayya, Yashwanth
AU - Keerthikumar, Shivakumar
AU - Kashyap, Manoj Kr
AU - Goel, Renu
AU - Balakrishnan, Lavanya
AU - Dwivedi, Sutopa
AU - Pathare, Swapnali
AU - Dikshit, Jyoti Bajpai
AU - Maharudraiah, Jagadeesha
AU - Singh, Sujay
AU - Sameer Kumar, Ghantasala S.
AU - Vijayakumar, M.
AU - Kumar, Kariyanakatte Veeraiah Veerendra
AU - Premalatha, Chennagiri Shrinivasamurthy
AU - Tata, Pramila
AU - Hariharan, Ramesh
AU - Roa, Juan Carlos
AU - Prasad, T. S.K.
AU - Chaerkady, Raghothama
AU - Rekha, Vijay Kr
AU - Pandey, Akhilesh
PY - 2011
Y1 - 2011
N2 - Gastric cancer is the second leading cause of cancer death worldwide, both in men and women. A genomewide gene expression analysis was carried out to identify differentially expressed genes in gastric adenocarcinoma tissues as compared to adjacent normal tissues. We used Agilent's whole human genome oligonucleotide microarray platform representing ~41,000 genes to carry gene expression analysis. Two-color microarray analysis was employed to directly compare the expression of genes between tumor and normal tissues. Through this approach, we identified several previously known candidate genes along with a number of novel candidate genes in gastric cancer. Testican-1 (SPOCK1) was one of the novel molecules that was 10-fold upregulated in tumors. Using tissue microarrays, we validated the expression of testican-1 by immunohistochemical staining. It was overexpressed in 56% (160/282) of the cases tested. Pathway analysis led to the identification of several networks in which SPOCK1 was among the topmost networks of interacting genes. By gene enrichment analysis, we identified several genes involved in cell adhesion and cell proliferation to be significantly upregulated while those corresponding to metabolic pathways were significantly downregulated. The differentially expressed genes identified in this study are candidate biomarkers for gastric adenoacarcinoma.
AB - Gastric cancer is the second leading cause of cancer death worldwide, both in men and women. A genomewide gene expression analysis was carried out to identify differentially expressed genes in gastric adenocarcinoma tissues as compared to adjacent normal tissues. We used Agilent's whole human genome oligonucleotide microarray platform representing ~41,000 genes to carry gene expression analysis. Two-color microarray analysis was employed to directly compare the expression of genes between tumor and normal tissues. Through this approach, we identified several previously known candidate genes along with a number of novel candidate genes in gastric cancer. Testican-1 (SPOCK1) was one of the novel molecules that was 10-fold upregulated in tumors. Using tissue microarrays, we validated the expression of testican-1 by immunohistochemical staining. It was overexpressed in 56% (160/282) of the cases tested. Pathway analysis led to the identification of several networks in which SPOCK1 was among the topmost networks of interacting genes. By gene enrichment analysis, we identified several genes involved in cell adhesion and cell proliferation to be significantly upregulated while those corresponding to metabolic pathways were significantly downregulated. The differentially expressed genes identified in this study are candidate biomarkers for gastric adenoacarcinoma.
KW - DNA microarray
KW - Gastric cancer
KW - GeneSpring GX
KW - Immunohistochemistry
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U2 - 10.4172/jpb.1000170
DO - 10.4172/jpb.1000170
M3 - Article
AN - SCOPUS:79955496492
SN - 0974-276X
VL - 4
SP - 74
EP - 82
JO - Journal of Proteomics and Bioinformatics
JF - Journal of Proteomics and Bioinformatics
IS - 4
ER -