Gene expression profiling in human lung development: An abundant resource for lung adenocarcinoma prognosis

Lin Feng, Jiamei Wang, Bangrong Cao, Yi Zhang, Bo Wu, Xuebing Di, Wei Jiang, Ning An, Dan Lu, Suhong Gao, Yuda Zhao, Zhaoli Chen, Yousheng Mao, Yanning Gao, Deshan Zhou, Jin Jen, Xiaohong Liu, Yunping Zhang, Xia Li, Kaitai ZhangJie He, Shujun Cheng

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14 Scopus citations

Abstract

A tumor can be viewed as a special "organ" that undergoes aberrant and poorly regulated organogenesis. Progress in cancer prognosis and therapy might be facilitated by re-examining distinctive processes that operate during normal development, to elucidate the intrinsic features of cancer that are significantly obscured by its heterogeneity. The global gene expression signatures of 44 human lung tissues at four development stages from Asian descent and 69 lung adenocarcinoma (ADC) tissue samples from ethnic Chinese patients were profiled using microarrays. All of the genes were classified into 27 distinct groups based on their expression patterns (named as PTN1 to PTN27) during the developmental process. In lung ADC, genes whose expression levels decreased steadily during lung development (genes in PTN1) generally had their expression reactivated, while those with uniformly increasing expression levels (genes in PTN27) had their expression suppressed. The genes in PTN1 contain many n-gene signatures that are of prognostic value for lung ADC. The prognostic relevance of a 12-gene demonstrator for patient survival was characterized in five cohorts of healthy and ADC patients [ADC-CICAMS (n = 69, p = 0.007), ADC-PNAS (n = 125, p = 0.0063), ADC-GSE13213 (n = 117, p = 0.0027), ADC-GSE8894 (n = 62, p = 0.01), and ADC-NCI (n = 282, p = 0.045)] and in four groups of stage I patients [ADC-CICAMS (n = 22, p = 0.017), ADC-PNAS (n = 76, p = 0.018), ADC-GSE13213 (n = 79, p = 0.02), and ADC-qPCR (n = 62, p = 0.006)]. In conclusion, by comparison of gene expression profiles during human lung developmental process and lung ADC progression, we revealed that the genes with a uniformly decreasing expression pattern during lung development are of enormous prognostic value for lung ADC.

Original languageEnglish (US)
Article numbere105639
JournalPLoS One
Volume9
Issue number8
DOIs
StatePublished - Aug 20 2014

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ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Feng, L., Wang, J., Cao, B., Zhang, Y., Wu, B., Di, X., Jiang, W., An, N., Lu, D., Gao, S., Zhao, Y., Chen, Z., Mao, Y., Gao, Y., Zhou, D., Jen, J., Liu, X., Zhang, Y., Li, X., ... Cheng, S. (2014). Gene expression profiling in human lung development: An abundant resource for lung adenocarcinoma prognosis. PLoS One, 9(8), [e105639]. https://doi.org/10.1371/journal.pone.0105639