Gene expression profiling in human insulinoma tissue: Genes involved in the insulin secretion pathway and cloning of novel full-length cDNAs

X. C. Wang, S. Y. Xu, Xinyan Wu, H. D. Song, Y. F. Mao, H. Y. Fan, F. Yu, B. Mou, Y. Y. Gu, L. Q. Xu, X. O. Zhou, Z. Chen, J. L. Chen, R. M. Hu

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Insulinoma is a clinically common cause of organic hypoglycemia. The prominent characteristic of insulinoma is endogenous hyperinsulinism. Until now, the molecular biology of human insulinoma has been little understood. In this study, gene expression profiling of human insulinoma was established by expressed sequence tag (EST) sequencing and cDNA array. A total of 2063 clones were obtained, of these, 1589 clones were derived from EST sequencing, 975 clones were derived from cDNA array and 501 clones were shared by the two methods. G protein α-stimulating activity polypeptide (Gsα) and carboxypeptidase E (CPE) were the most highly expressed genes in human insulinoma, as derived by EST sequencing and cDNA array respectively. The genes involved in the protein/insulin secretion pathway were strongly expressed in human insulinoma tissue. Meanwhile, eight full-length cDNAs of novel genes were cloned and sequenced. The results demonstrated the molecular biology of human insulinoma tissue at the level of transcript abundance and validated the efficacy of EST sequencing combined with cDNA array in the construction of gene expression profiling. In conclusion, the predominance of the genes participating in the secretory pathway suggested that regulation of secretion might be a major mechanism by which insulin release is abnormally increased in patients with insulinomas. It was also concluded that overexpression of the Gsα gene played an important role in the pathogenesis of insulinoma.

Original languageEnglish (US)
Pages (from-to)295-303
Number of pages9
JournalEndocrine-Related Cancer
Volume11
Issue number2
DOIs
StatePublished - Jun 1 2004
Externally publishedYes

Fingerprint

Insulinoma
Secretory Pathway
Gene Expression Profiling
Organism Cloning
Complementary DNA
Insulin
Expressed Sequence Tags
Genes
Oligonucleotide Array Sequence Analysis
Clone Cells
Molecular Biology
Carboxypeptidase H
Hyperinsulinism
GTP-Binding Proteins
Hypoglycemia
Peptides

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Oncology
  • Endocrinology
  • Cancer Research

Cite this

Gene expression profiling in human insulinoma tissue : Genes involved in the insulin secretion pathway and cloning of novel full-length cDNAs. / Wang, X. C.; Xu, S. Y.; Wu, Xinyan; Song, H. D.; Mao, Y. F.; Fan, H. Y.; Yu, F.; Mou, B.; Gu, Y. Y.; Xu, L. Q.; Zhou, X. O.; Chen, Z.; Chen, J. L.; Hu, R. M.

In: Endocrine-Related Cancer, Vol. 11, No. 2, 01.06.2004, p. 295-303.

Research output: Contribution to journalArticle

Wang, XC, Xu, SY, Wu, X, Song, HD, Mao, YF, Fan, HY, Yu, F, Mou, B, Gu, YY, Xu, LQ, Zhou, XO, Chen, Z, Chen, JL & Hu, RM 2004, 'Gene expression profiling in human insulinoma tissue: Genes involved in the insulin secretion pathway and cloning of novel full-length cDNAs', Endocrine-Related Cancer, vol. 11, no. 2, pp. 295-303. https://doi.org/10.1677/erc.0.0110295
Wang, X. C. ; Xu, S. Y. ; Wu, Xinyan ; Song, H. D. ; Mao, Y. F. ; Fan, H. Y. ; Yu, F. ; Mou, B. ; Gu, Y. Y. ; Xu, L. Q. ; Zhou, X. O. ; Chen, Z. ; Chen, J. L. ; Hu, R. M. / Gene expression profiling in human insulinoma tissue : Genes involved in the insulin secretion pathway and cloning of novel full-length cDNAs. In: Endocrine-Related Cancer. 2004 ; Vol. 11, No. 2. pp. 295-303.
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