Gene expression profile comparison of Barrett's esophagus epithelial cell cultures and biopsies

Jantine W P M Van Baal, A. M. Rygiel, F. Milano, M. Anderson, J. J G H M Bergman, C. A. Spek, Kenneth Ke Ning Wang, M. P. Peppelenbosch, K. K. Krishnadath

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Barrett's esophagus (BE) is a metaplastic process in which the normal squamous epithelium of the distal esophagus is replaced by columnar lined epithelium. The aim was to gain more insight in the process of metaplasia and to identify which genes are specifically expressed by the epithelial cells and the surrounding tissues in BE. Hereto, the gene expression profile of a BE epithelial primary cell culture was compared to that of a BE biopsy. To specifically obtain the epithelial cell layer, epithelial cells from biopsies of BE were cultured using a Barrett specific culturing medium. Serial analysis of gene expression was applied to obtain a transcription library of the primary epithelial cell culture. The transcriptome was analyzed and compared to a previously described transcriptome of a BE biopsy. Validation of results by reverse transcriptase-polymerase chain reaction was performed using tissues of 16 BE patients and 16 primary cell cultures. Over 43000 tags were sequenced. Genes specifically expressed by the Barrett epithelial cells were for instance Lipocalin 2 and Cyclin D1, whereas annexin A10, trefoil factor (TFF)1 and TFF2 were specifically expressed in the BE biopsies. The comparison of the gene expression profiles of BE primary cultured epithelial cells with BE biopsy defines a subset of genes that are specifically expressed by the epithelial cells and another subset that most likely is expressed by the underlying stromal tissues in the BE biopsy specimens.

Original languageEnglish (US)
Pages (from-to)628-633
Number of pages6
JournalDiseases of the Esophagus
Volume21
Issue number7
DOIs
StatePublished - 2008

Fingerprint

Barrett Esophagus
Transcriptome
Cell Culture Techniques
Epithelial Cells
Biopsy
Primary Cell Culture
Epithelium
Genes
Annexins
Cyclin D1
antineoplaston A10
Metaplasia
Reverse Transcriptase Polymerase Chain Reaction
Esophagus
Cultured Cells
Gene Expression

Keywords

  • Barrett's esophagus
  • Geneexpression profile
  • Primarycell culture
  • Serialanalysis of gene expression
  • Transcriptome

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Van Baal, J. W. P. M., Rygiel, A. M., Milano, F., Anderson, M., Bergman, J. J. G. H. M., Spek, C. A., ... Krishnadath, K. K. (2008). Gene expression profile comparison of Barrett's esophagus epithelial cell cultures and biopsies. Diseases of the Esophagus, 21(7), 628-633. https://doi.org/10.1111/j.1442-2050.2008.00810.x

Gene expression profile comparison of Barrett's esophagus epithelial cell cultures and biopsies. / Van Baal, Jantine W P M; Rygiel, A. M.; Milano, F.; Anderson, M.; Bergman, J. J G H M; Spek, C. A.; Wang, Kenneth Ke Ning; Peppelenbosch, M. P.; Krishnadath, K. K.

In: Diseases of the Esophagus, Vol. 21, No. 7, 2008, p. 628-633.

Research output: Contribution to journalArticle

Van Baal, JWPM, Rygiel, AM, Milano, F, Anderson, M, Bergman, JJGHM, Spek, CA, Wang, KKN, Peppelenbosch, MP & Krishnadath, KK 2008, 'Gene expression profile comparison of Barrett's esophagus epithelial cell cultures and biopsies', Diseases of the Esophagus, vol. 21, no. 7, pp. 628-633. https://doi.org/10.1111/j.1442-2050.2008.00810.x
Van Baal, Jantine W P M ; Rygiel, A. M. ; Milano, F. ; Anderson, M. ; Bergman, J. J G H M ; Spek, C. A. ; Wang, Kenneth Ke Ning ; Peppelenbosch, M. P. ; Krishnadath, K. K. / Gene expression profile comparison of Barrett's esophagus epithelial cell cultures and biopsies. In: Diseases of the Esophagus. 2008 ; Vol. 21, No. 7. pp. 628-633.
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