Gender differences in the effect of genistein on vascular smooth muscle cells: A possible cardioprotective effect?

A. Vincent, M. Ruan, L. A. Fitzpatrick

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective: Isoflavones are a class of phytoestrogens found abundantly in soybeans. They share structural similarity to 17-beta-estradiol, bind to the estrogen receptors alpha and beta, and produce estrogenic or antiestrogenic effects. Atherosclerosis is an inflammatory-mediated fibroproliferative response to injury to the arterial wall. Vascular smooth muscle cells (VSMCs) are the prominent cells in the atherosclerotic plaque. VSMCs contain estrogen receptors and, at physiologic concentrations, 17-beta-estradiol-inhibited proliferation of VSMCs from sexually mature female pigs. We determined if genistein inhibited proliferation and altered matrix protein production in VSMCs from coronary arteries of sexually mature pigs. Methods: The effect of genistein on cell proliferation was assessed by thymidine incorporation. The effect of genistein on matrix protein production in VSMCs was assessed by Western blot analysis. Results and Conclusion: We demonstrate gender-specific effects in the proliferation of coronary artery vascular smooth muscle cells obtained from a sexually mature pig model treated with genistein at physiologically relevant concentrations. There were no differences in the amount of estrogen receptor proteins between the genders, suggesting that nongenomic mechanisms may be responsible for these effects. Genistein also upregulated matrix protein expression, which may be related to the formation of the atherosclerotic plaque. Overall, these results suggest possible cardioprotection by genistein.

Original languageEnglish (US)
Pages (from-to)28-34
Number of pages7
JournalJournal of Gender-Specific Medicine
Volume4
Issue number1
StatePublished - 2001

Fingerprint

Genistein
Vascular Smooth Muscle
Smooth Muscle Myocytes
Swine
Atherosclerotic Plaques
Estradiol
Coronary Vessels
Phytoestrogens
Estrogen Receptor beta
Proteins
Isoflavones
Estrogen Receptor alpha
Soybeans
Estrogen Receptors
Thymidine
Atherosclerosis
Western Blotting
Cell Proliferation
Wounds and Injuries

ASJC Scopus subject areas

  • Physiology
  • Pathology and Forensic Medicine

Cite this

Gender differences in the effect of genistein on vascular smooth muscle cells : A possible cardioprotective effect? / Vincent, A.; Ruan, M.; Fitzpatrick, L. A.

In: Journal of Gender-Specific Medicine, Vol. 4, No. 1, 2001, p. 28-34.

Research output: Contribution to journalArticle

@article{72a51850adc0455fa89a74d53cf74109,
title = "Gender differences in the effect of genistein on vascular smooth muscle cells: A possible cardioprotective effect?",
abstract = "Objective: Isoflavones are a class of phytoestrogens found abundantly in soybeans. They share structural similarity to 17-beta-estradiol, bind to the estrogen receptors alpha and beta, and produce estrogenic or antiestrogenic effects. Atherosclerosis is an inflammatory-mediated fibroproliferative response to injury to the arterial wall. Vascular smooth muscle cells (VSMCs) are the prominent cells in the atherosclerotic plaque. VSMCs contain estrogen receptors and, at physiologic concentrations, 17-beta-estradiol-inhibited proliferation of VSMCs from sexually mature female pigs. We determined if genistein inhibited proliferation and altered matrix protein production in VSMCs from coronary arteries of sexually mature pigs. Methods: The effect of genistein on cell proliferation was assessed by thymidine incorporation. The effect of genistein on matrix protein production in VSMCs was assessed by Western blot analysis. Results and Conclusion: We demonstrate gender-specific effects in the proliferation of coronary artery vascular smooth muscle cells obtained from a sexually mature pig model treated with genistein at physiologically relevant concentrations. There were no differences in the amount of estrogen receptor proteins between the genders, suggesting that nongenomic mechanisms may be responsible for these effects. Genistein also upregulated matrix protein expression, which may be related to the formation of the atherosclerotic plaque. Overall, these results suggest possible cardioprotection by genistein.",
author = "A. Vincent and M. Ruan and Fitzpatrick, {L. A.}",
year = "2001",
language = "English (US)",
volume = "4",
pages = "28--34",
journal = "Journal of Gender-Specific Medicine",
issn = "1523-7036",
publisher = "MultiMedia Healthcare Inc.",
number = "1",

}

TY - JOUR

T1 - Gender differences in the effect of genistein on vascular smooth muscle cells

T2 - A possible cardioprotective effect?

AU - Vincent, A.

AU - Ruan, M.

AU - Fitzpatrick, L. A.

PY - 2001

Y1 - 2001

N2 - Objective: Isoflavones are a class of phytoestrogens found abundantly in soybeans. They share structural similarity to 17-beta-estradiol, bind to the estrogen receptors alpha and beta, and produce estrogenic or antiestrogenic effects. Atherosclerosis is an inflammatory-mediated fibroproliferative response to injury to the arterial wall. Vascular smooth muscle cells (VSMCs) are the prominent cells in the atherosclerotic plaque. VSMCs contain estrogen receptors and, at physiologic concentrations, 17-beta-estradiol-inhibited proliferation of VSMCs from sexually mature female pigs. We determined if genistein inhibited proliferation and altered matrix protein production in VSMCs from coronary arteries of sexually mature pigs. Methods: The effect of genistein on cell proliferation was assessed by thymidine incorporation. The effect of genistein on matrix protein production in VSMCs was assessed by Western blot analysis. Results and Conclusion: We demonstrate gender-specific effects in the proliferation of coronary artery vascular smooth muscle cells obtained from a sexually mature pig model treated with genistein at physiologically relevant concentrations. There were no differences in the amount of estrogen receptor proteins between the genders, suggesting that nongenomic mechanisms may be responsible for these effects. Genistein also upregulated matrix protein expression, which may be related to the formation of the atherosclerotic plaque. Overall, these results suggest possible cardioprotection by genistein.

AB - Objective: Isoflavones are a class of phytoestrogens found abundantly in soybeans. They share structural similarity to 17-beta-estradiol, bind to the estrogen receptors alpha and beta, and produce estrogenic or antiestrogenic effects. Atherosclerosis is an inflammatory-mediated fibroproliferative response to injury to the arterial wall. Vascular smooth muscle cells (VSMCs) are the prominent cells in the atherosclerotic plaque. VSMCs contain estrogen receptors and, at physiologic concentrations, 17-beta-estradiol-inhibited proliferation of VSMCs from sexually mature female pigs. We determined if genistein inhibited proliferation and altered matrix protein production in VSMCs from coronary arteries of sexually mature pigs. Methods: The effect of genistein on cell proliferation was assessed by thymidine incorporation. The effect of genistein on matrix protein production in VSMCs was assessed by Western blot analysis. Results and Conclusion: We demonstrate gender-specific effects in the proliferation of coronary artery vascular smooth muscle cells obtained from a sexually mature pig model treated with genistein at physiologically relevant concentrations. There were no differences in the amount of estrogen receptor proteins between the genders, suggesting that nongenomic mechanisms may be responsible for these effects. Genistein also upregulated matrix protein expression, which may be related to the formation of the atherosclerotic plaque. Overall, these results suggest possible cardioprotection by genistein.

UR - http://www.scopus.com/inward/record.url?scp=0035003171&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035003171&partnerID=8YFLogxK

M3 - Article

C2 - 11324237

AN - SCOPUS:0035003171

VL - 4

SP - 28

EP - 34

JO - Journal of Gender-Specific Medicine

JF - Journal of Gender-Specific Medicine

SN - 1523-7036

IS - 1

ER -