GATA-3 dominant negative mutant. Functional redundancy of the T cell receptor α and β enhancers

V. M. Smith, P. P. Lee, S. Szychowski, A. Winoto

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

The GATA family of transcription factors regulates a wide variety of genes, including those involved in differentiation of erythrocytes and T lymphocytes. We report here the creation of a dominant negative mutant of GATA-3, KRR, which effectively blocks wild-type GATA-1, GATA-2, and GATA-3 transactivation when coexpressed in transient assays. KRR was generated by site-directed mutagenesis while investigating a putative activation domain of GATA-3, located between its two zinc fingers. The GATA-3 KRR mutation does not affect expression, nuclear translocation, or the ability to bind to a consensus GATA sequence. KRR can suppress the activity of the minimal T cell receptor (TCR) α and β enhancers by 12- and 3.4-fold, respectively. However, KRR did not have a significant effect on the activity of larger TCR- α and β enhancer fragments. Thus, functional redundancy in the TCR-α and β enhancers can compensate for the loss of GATA-3 activity.

Original languageEnglish (US)
Pages (from-to)1515-1520
Number of pages6
JournalJournal of Biological Chemistry
Volume270
Issue number4
DOIs
StatePublished - 1995

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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