Abstract
Objectives: A subset of colon cancers originates from sessile serrated adenomas/polyps (SSA/Ps). Our goal was to identify markers for SSA/Ps that could aid in distinguishing them from hyperplastic polyps (HPs). Methods: We performed immunostaining for gastric proteins MUC5AC and TFF1 in formalin-fixed, paraffinembedded (FFPE) samples of HPs (n=47), SSA/Ps (n=37), and normal colon (n=30). Results: Control mucosa expressed only trace amounts of MUC5AC and TFF1. HPs exhibited an 11.3- and 11.4-fold increase in MUC5AC and TFF1 expression confined to the upper segments of the crypts near the luminal surface of the polyps. SSA/Ps displayed on average 1.6-fold (MUC5AC, P<.008) and 1.4-fold (TFF1, P<.03) higher signal intensity for these markers than HPs, with a dramatic coexpression of MUC5AC and TFF1 typically occupying the entire length of the crypt. Immunoperoxidase results were similar to immunofluorescence staining for both MUC5AC and TFF1. Conclusions: Our results suggest that the analysis of expression of MUC5AC and TFF1 may be useful for differentiating SSA/Ps from HPs. We also suggest the possibility that crypt morphology may be at least partly due to overproduction of highly viscous gastric mucins and that these proteins may play a role in the serrated pathway to colon carcinogenesis.
Original language | English (US) |
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Pages (from-to) | 530-537 |
Number of pages | 8 |
Journal | American journal of clinical pathology |
Volume | 146 |
Issue number | 5 |
DOIs | |
State | Published - 2016 |
Keywords
- Colon cancer
- Hyperplastic polyps
- MUC5AC
- Serrated polyps
- Sessile serrated adenomas/polyps
- TFF1
ASJC Scopus subject areas
- Pathology and Forensic Medicine