Gastric proteins MUC5AC and TFF1 as potential diagnostic markers of colonic sessile serrated adenomas/polyps

Magomed Khaidakov, Keith K. Lai, D. Roudachevski, Julietta Sargsyan, Hannah E. Goyne, Rish K. Pai, Laura W. Lamps, Curt H. Hagedorn

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Objectives: A subset of colon cancers originates from sessile serrated adenomas/polyps (SSA/Ps). Our goal was to identify markers for SSA/Ps that could aid in distinguishing them from hyperplastic polyps (HPs). Methods: We performed immunostaining for gastric proteins MUC5AC and TFF1 in formalin-fixed, paraffinembedded (FFPE) samples of HPs (n=47), SSA/Ps (n=37), and normal colon (n=30). Results: Control mucosa expressed only trace amounts of MUC5AC and TFF1. HPs exhibited an 11.3- and 11.4-fold increase in MUC5AC and TFF1 expression confined to the upper segments of the crypts near the luminal surface of the polyps. SSA/Ps displayed on average 1.6-fold (MUC5AC, P<.008) and 1.4-fold (TFF1, P<.03) higher signal intensity for these markers than HPs, with a dramatic coexpression of MUC5AC and TFF1 typically occupying the entire length of the crypt. Immunoperoxidase results were similar to immunofluorescence staining for both MUC5AC and TFF1. Conclusions: Our results suggest that the analysis of expression of MUC5AC and TFF1 may be useful for differentiating SSA/Ps from HPs. We also suggest the possibility that crypt morphology may be at least partly due to overproduction of highly viscous gastric mucins and that these proteins may play a role in the serrated pathway to colon carcinogenesis.

Original languageEnglish (US)
Pages (from-to)530-537
Number of pages8
JournalAmerican journal of clinical pathology
Volume146
Issue number5
DOIs
StatePublished - 2016

Keywords

  • Colon cancer
  • Hyperplastic polyps
  • MUC5AC
  • Serrated polyps
  • Sessile serrated adenomas/polyps
  • TFF1

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Khaidakov, M., Lai, K. K., Roudachevski, D., Sargsyan, J., Goyne, H. E., Pai, R. K., Lamps, L. W., & Hagedorn, C. H. (2016). Gastric proteins MUC5AC and TFF1 as potential diagnostic markers of colonic sessile serrated adenomas/polyps. American journal of clinical pathology, 146(5), 530-537. https://doi.org/10.1093/AJCP/AQW142