TY - JOUR
T1 - Gallbladder CCK receptors
T2 - species differences in glycosylation of similar protein cores
AU - Schjoldager, Birgit
AU - Molero, Xavier
AU - Miller, Laurence J.
N1 - Funding Information:
The authors thank Dr. Stephen Powers and Delia Pinon for providing synthetic peptides, Elizabeth Hadac for excellent technical assistance, and Marilyn LeQve for typing the manuscript. This work was supported by grants from the National Institutes of Health (DK-32878 and DK-34988) and from the Chadwick Foundation.
PY - 1990/5/21
Y1 - 1990/5/21
N2 - Receptors for cholecystokinin (CCK) on gallbladder muscularis smooth muscle have different apparent sizes in man (Mr = 85,000-95,000) and cow (Mr = 70,000-85,000). In this work, these receptors were demonstrated to represent N-linked complex glycoproteins with Mr = 43,000 protein cores, based on lectin-affinity chromatography and the deglycosylation of bands affinity labeled with 125I-d-Tyr-Gly-[(Nle28,31, pNO2--Phe33)CCK-26-33] using neuraminidase, O-glycanase and endoglycosidases H and F. Similarities in the core proteins were further demonstrated by Staphylococcus aureus V8 protease peptide mapping, in which both proteins yielded similar fragment patterns. Thus, gallbladder CCK receptors present in man and cow are both N-linked complex glycoproteins, with different carbohydrate domains and similar protein cores.
AB - Receptors for cholecystokinin (CCK) on gallbladder muscularis smooth muscle have different apparent sizes in man (Mr = 85,000-95,000) and cow (Mr = 70,000-85,000). In this work, these receptors were demonstrated to represent N-linked complex glycoproteins with Mr = 43,000 protein cores, based on lectin-affinity chromatography and the deglycosylation of bands affinity labeled with 125I-d-Tyr-Gly-[(Nle28,31, pNO2--Phe33)CCK-26-33] using neuraminidase, O-glycanase and endoglycosidases H and F. Similarities in the core proteins were further demonstrated by Staphylococcus aureus V8 protease peptide mapping, in which both proteins yielded similar fragment patterns. Thus, gallbladder CCK receptors present in man and cow are both N-linked complex glycoproteins, with different carbohydrate domains and similar protein cores.
KW - Cholecystokinin receptor
KW - Glycosylation
KW - Peptide hormone receptor
KW - Species difference
KW - smooth muscle
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U2 - 10.1016/0167-0115(90)90024-Q
DO - 10.1016/0167-0115(90)90024-Q
M3 - Article
C2 - 2377742
AN - SCOPUS:0025231280
SN - 0167-0115
VL - 28
SP - 265
EP - 272
JO - Regulatory Peptides
JF - Regulatory Peptides
IS - 3
ER -