Gallbladder CCK receptors: species differences in glycosylation of similar protein cores

Birgit Schjoldager, Xavier Molero, Laurence J. Miller

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Receptors for cholecystokinin (CCK) on gallbladder muscularis smooth muscle have different apparent sizes in man (Mr = 85,000-95,000) and cow (Mr = 70,000-85,000). In this work, these receptors were demonstrated to represent N-linked complex glycoproteins with Mr = 43,000 protein cores, based on lectin-affinity chromatography and the deglycosylation of bands affinity labeled with 125I-d-Tyr-Gly-[(Nle28,31, pNO2--Phe33)CCK-26-33] using neuraminidase, O-glycanase and endoglycosidases H and F. Similarities in the core proteins were further demonstrated by Staphylococcus aureus V8 protease peptide mapping, in which both proteins yielded similar fragment patterns. Thus, gallbladder CCK receptors present in man and cow are both N-linked complex glycoproteins, with different carbohydrate domains and similar protein cores.

Original languageEnglish (US)
Pages (from-to)265-272
Number of pages8
JournalRegulatory Peptides
Volume28
Issue number3
DOIs
StatePublished - May 21 1990

Keywords

  • Cholecystokinin receptor
  • Glycosylation
  • Peptide hormone receptor
  • Species difference
  • smooth muscle

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

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