Galectin-3 stimulates preadipocyte proliferation and is up-regulated in growing adipose tissue

Kohji Kiwaki, Colleen M. Novak, Daniel K. Hsu, Fu Tong Liu, James A. Levine

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Objective: Some cytokines and mediators of inflammation can alter adiposity through their effects on adipocyte number. To probe the molecular basis of obesity, this study determined whether galectin-3 was present in adipose tissue and investigated its effects on fat cell number. Research Methods and Procedures: In the first study, obesity-prone C57BL/6J mice were fed with high-fat (58%) diet. Epididymal fat pads were collected at Day 0, Day 60, and Day 120 after the start of high-fat feeding. Results: Levels of adipocyte galectin-3 protein, determined using Western blot analysis, increased as the mice became obese. Galectin-3 mRNA and protein were then detected in human adipose tissue, primarily in the preadipocyte fraction. It was found that recombinant human galectin-3 stimulated proliferation of primary cultured preadipocytes as well as DNA synthesis through lectin-carbohydrate interaction. Discussion: Galectin-3, which has been known to play a versatile role especially in immune cells, might play a role also in adipose tissue and be associated with the pathophysiology of obesity.

Original languageEnglish (US)
Pages (from-to)32-39
Number of pages8
JournalObesity
Volume15
Issue number1
DOIs
StatePublished - Jan 2007

Fingerprint

Galectin 3
Adipose Tissue
Adipocytes
Obesity
Molecular Probes
Inflammation Mediators
Obese Mice
Adiposity
High Fat Diet
Inbred C57BL Mouse
Lectins
Proteins
Cell Count
Western Blotting
Fats
Carbohydrates
Cytokines
Messenger RNA
DNA
Research

Keywords

  • C57BL/6J mice
  • Diet
  • Obesity phenotypes

ASJC Scopus subject areas

  • Endocrinology
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Kiwaki, K., Novak, C. M., Hsu, D. K., Liu, F. T., & Levine, J. A. (2007). Galectin-3 stimulates preadipocyte proliferation and is up-regulated in growing adipose tissue. Obesity, 15(1), 32-39. https://doi.org/10.1038/oby.2007.526

Galectin-3 stimulates preadipocyte proliferation and is up-regulated in growing adipose tissue. / Kiwaki, Kohji; Novak, Colleen M.; Hsu, Daniel K.; Liu, Fu Tong; Levine, James A.

In: Obesity, Vol. 15, No. 1, 01.2007, p. 32-39.

Research output: Contribution to journalArticle

Kiwaki, Kohji ; Novak, Colleen M. ; Hsu, Daniel K. ; Liu, Fu Tong ; Levine, James A. / Galectin-3 stimulates preadipocyte proliferation and is up-regulated in growing adipose tissue. In: Obesity. 2007 ; Vol. 15, No. 1. pp. 32-39.
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