G-protein-dependent cell surface dynamics of the human serotonin 1A receptor tagged to yellow fluorescent protein

Thomas J. Pucadyil, Shanti Kalipatnapu, Kaleeckal G. Harikumar, Nandini Rangaraj, Sadashiva S. Karnik, Amitabha Chattopadhyay

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Serotonergic signaling appears to play a key role in the generation and modulation of various cognitive, behavioral, and developmental processes. The serotonin1A receptor is an important member of the superfamily of seven transmembrane domain G-protein-coupled receptors and is the most extensively studied among the serotonin receptors. Several aspects of serotonin1A receptor biology such as cellular distribution and signal transduction characteristics are technically difficult to address in living cells on account of the inability to optically track these receptors with fluorescence-based techniques. We describe here the characterization of the serotonin1A receptor tagged to the enhanced yellow fluorescent protein (EYFP) stably expressed in Chinese hamster ovary (CHO) cells. These receptors were found to be essentially similar to the native receptor in pharmacological assays and can therefore be used to reliably explore aspects of receptor biology such as cellular distribution and dynamics on account of their intrinsic fluorescent properties. Analysis of the cell surface dynamics of these receptors by fluorescence recovery after photobleaching (FRAP) experiments has provided novel insight into the molecular mechanism of signal transduction of serotonin1A receptors in living cells. Interestingly, addition of pharmacologically well-characterized ligands or activators of G-proteins altered the diffusion characteristics of the receptor in a manner consistent with the G-protein activation model. These results demonstrate, for the first time, that membrane dynamics of this receptor is modulated in a G-protein-dependent manner.

Original languageEnglish (US)
Pages (from-to)15852-15862
Number of pages11
JournalBiochemistry
Volume43
Issue number50
DOIs
StatePublished - Dec 21 2004

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Receptor, Serotonin, 5-HT1A
GTP-Binding Proteins
Signal transduction
Cells
Signal Transduction
Fluorescence
Fluorescence Recovery After Photobleaching
Photobleaching
Proteins
Serotonin Receptors
Cell Surface Receptors
G-Protein-Coupled Receptors
Cricetulus
Ovary
Assays
Chemical activation
Modulation
Pharmacology
Ligands
Membranes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Pucadyil, T. J., Kalipatnapu, S., Harikumar, K. G., Rangaraj, N., Karnik, S. S., & Chattopadhyay, A. (2004). G-protein-dependent cell surface dynamics of the human serotonin 1A receptor tagged to yellow fluorescent protein. Biochemistry, 43(50), 15852-15862. https://doi.org/10.1021/bi0480887

G-protein-dependent cell surface dynamics of the human serotonin 1A receptor tagged to yellow fluorescent protein. / Pucadyil, Thomas J.; Kalipatnapu, Shanti; Harikumar, Kaleeckal G.; Rangaraj, Nandini; Karnik, Sadashiva S.; Chattopadhyay, Amitabha.

In: Biochemistry, Vol. 43, No. 50, 21.12.2004, p. 15852-15862.

Research output: Contribution to journalArticle

Pucadyil, TJ, Kalipatnapu, S, Harikumar, KG, Rangaraj, N, Karnik, SS & Chattopadhyay, A 2004, 'G-protein-dependent cell surface dynamics of the human serotonin 1A receptor tagged to yellow fluorescent protein', Biochemistry, vol. 43, no. 50, pp. 15852-15862. https://doi.org/10.1021/bi0480887
Pucadyil TJ, Kalipatnapu S, Harikumar KG, Rangaraj N, Karnik SS, Chattopadhyay A. G-protein-dependent cell surface dynamics of the human serotonin 1A receptor tagged to yellow fluorescent protein. Biochemistry. 2004 Dec 21;43(50):15852-15862. https://doi.org/10.1021/bi0480887
Pucadyil, Thomas J. ; Kalipatnapu, Shanti ; Harikumar, Kaleeckal G. ; Rangaraj, Nandini ; Karnik, Sadashiva S. ; Chattopadhyay, Amitabha. / G-protein-dependent cell surface dynamics of the human serotonin 1A receptor tagged to yellow fluorescent protein. In: Biochemistry. 2004 ; Vol. 43, No. 50. pp. 15852-15862.
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