Fusion of dynactin 1 to anaplastic lymphoma kinase in inflammatory myofibroblastic tumor

Xiaoke Wang, Chandra Krishnan, Edward P. Nguyen, Kevin J. Meyer, Jennifer Oliveira, Ping Yang, Eunhee S. Yi, Michele R. Erickson-Johnson, Michael J Yaszemski, Avudaiappan Maran, Andre M. Oliveira

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Inflammatory myofibroblastic tumor is an uncommon neoplasm that occurs more often in younger patients. Approximately 50% of inflammatory myofibroblastic tumors are characterized by anaplastic lymphoma kinase fusion genes, more commonly TPM3-anaplastic lymphoma kinase and TPM4-anaplastic lymphoma kinase. Herein, we report a novel fusion of dynactin 1 to anaplastic lymphoma kinase in a neck inflammatory myofibroblastic tumor diagnosed in a 7-year-old girl. Histologic evaluation showed a perineurioma-like bland spindle cell neoplasm with positive immunohistochemical staining for anaplastic lymphoma kinase, S-100, and CD34 but negative for epithelial membrane antigen. Standard cytogenetic analysis showed a der(2)t(2;12)(p23;q11). Fluorescence in situ hybridization demonstrated separation of the anaplastic lymphoma kinase locus. 5′-rapid amplification of complementary DNA ends polymerase chain reaction identified an in-frame fusion of dynactin 1 exon 16 on chromosome 2 to anaplastic lymphoma kinase exon 20. Reverse transcription-polymerase chain reaction with specific primers and direct sequencing confirmed the fusion. The structure of the fusion protein retains the cytoskeleton-associated protein-glycine domain and coiled coil domain of dynactin 1 and the receptor tyrosine kinase domain of anaplastic lymphoma kinase. This novel fusion gene is structurally similar to other previously described anaplastic lymphoma kinase fusion genes and may be associated with the unusual morphology and immunophenotype of this tumor.

Original languageEnglish (US)
Pages (from-to)2047-2052
Number of pages6
JournalHuman Pathology
Volume43
Issue number11
DOIs
StatePublished - Nov 2012

Fingerprint

Neoplasms
Gene Fusion
Exons
Nerve Sheath Neoplasms
Dynactin Complex
anaplastic lymphoma kinase
Mucin-1
Polymerase Chain Reaction
Chromosomes, Human, Pair 2
Cytogenetic Analysis
Receptor Protein-Tyrosine Kinases
DNA-Directed DNA Polymerase
Cytoskeleton
Fluorescence In Situ Hybridization
Glycine
Reverse Transcription
Neck
Complementary DNA
Staining and Labeling
Proteins

Keywords

  • ALK
  • DCTN1
  • Fusion
  • IMT

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Fusion of dynactin 1 to anaplastic lymphoma kinase in inflammatory myofibroblastic tumor. / Wang, Xiaoke; Krishnan, Chandra; Nguyen, Edward P.; Meyer, Kevin J.; Oliveira, Jennifer; Yang, Ping; Yi, Eunhee S.; Erickson-Johnson, Michele R.; Yaszemski, Michael J; Maran, Avudaiappan; Oliveira, Andre M.

In: Human Pathology, Vol. 43, No. 11, 11.2012, p. 2047-2052.

Research output: Contribution to journalArticle

Wang, X, Krishnan, C, Nguyen, EP, Meyer, KJ, Oliveira, J, Yang, P, Yi, ES, Erickson-Johnson, MR, Yaszemski, MJ, Maran, A & Oliveira, AM 2012, 'Fusion of dynactin 1 to anaplastic lymphoma kinase in inflammatory myofibroblastic tumor', Human Pathology, vol. 43, no. 11, pp. 2047-2052. https://doi.org/10.1016/j.humpath.2012.02.014
Wang, Xiaoke ; Krishnan, Chandra ; Nguyen, Edward P. ; Meyer, Kevin J. ; Oliveira, Jennifer ; Yang, Ping ; Yi, Eunhee S. ; Erickson-Johnson, Michele R. ; Yaszemski, Michael J ; Maran, Avudaiappan ; Oliveira, Andre M. / Fusion of dynactin 1 to anaplastic lymphoma kinase in inflammatory myofibroblastic tumor. In: Human Pathology. 2012 ; Vol. 43, No. 11. pp. 2047-2052.
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