Further development of a recombinant feline herpesvirus type 1 expressing the Gag protein of feline immunodeficiency virus

E. Sato, T. Miyazawa, Y. Nishimura, Y. Ikeda, K. Kawakami, M. Mochizuki, N. Yokoyama, K. Maeda, K. Fujita, M. Kohmoto, E. Takahashi, T. Mikami

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We have previously reported the construction of a recombinant feline herpesvirus type 1 (FHV-1), designated C7301ddlTK-gag, expressing the Gag precursor protein of feline immunodeficiency virus (FIV). In this study, we report the construction of a further recombinant FHV-1 (ddlTK(gBp)-gag) which carries an FHV-1 gB promoter sequence upstream of the FIV gag gene of C7301ddlTK-gag. Strong expression of the FIV Gag protein by ddlTK(gBp)-gag was confirmed by immunoblot analyses and enzyme-linked immunosorbent assays. Although C7301ddlTK-gag and ddlTK(gBp)-gag failed to induce anti-FIV Gag antibodies in cats, we confirmed the infectivity and stability of these recombinants in cats.

Original languageEnglish (US)
Pages (from-to)379-387
Number of pages9
JournalArchives of Virology
Volume146
Issue number2
DOIs
StatePublished - Apr 12 2001

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Further development of a recombinant feline herpesvirus type 1 expressing the Gag protein of feline immunodeficiency virus'. Together they form a unique fingerprint.

Cite this