TY - JOUR
T1 - Further development of a recombinant feline herpesvirus type 1 expressing the Gag protein of feline immunodeficiency virus
AU - Sato, E.
AU - Miyazawa, T.
AU - Nishimura, Y.
AU - Ikeda, Y.
AU - Kawakami, K.
AU - Mochizuki, M.
AU - Yokoyama, N.
AU - Maeda, K.
AU - Fujita, K.
AU - Kohmoto, M.
AU - Takahashi, E.
AU - Mikami, T.
PY - 2001
Y1 - 2001
N2 - We have previously reported the construction of a recombinant feline herpesvirus type 1 (FHV-1), designated C7301ddlTK-gag, expressing the Gag precursor protein of feline immunodeficiency virus (FIV). In this study, we report the construction of a further recombinant FHV-1 (ddlTK(gBp)-gag) which carries an FHV-1 gB promoter sequence upstream of the FIV gag gene of C7301ddlTK-gag. Strong expression of the FIV Gag protein by ddlTK(gBp)-gag was confirmed by immunoblot analyses and enzyme-linked immunosorbent assays. Although C7301ddlTK-gag and ddlTK(gBp)-gag failed to induce anti-FIV Gag antibodies in cats, we confirmed the infectivity and stability of these recombinants in cats.
AB - We have previously reported the construction of a recombinant feline herpesvirus type 1 (FHV-1), designated C7301ddlTK-gag, expressing the Gag precursor protein of feline immunodeficiency virus (FIV). In this study, we report the construction of a further recombinant FHV-1 (ddlTK(gBp)-gag) which carries an FHV-1 gB promoter sequence upstream of the FIV gag gene of C7301ddlTK-gag. Strong expression of the FIV Gag protein by ddlTK(gBp)-gag was confirmed by immunoblot analyses and enzyme-linked immunosorbent assays. Although C7301ddlTK-gag and ddlTK(gBp)-gag failed to induce anti-FIV Gag antibodies in cats, we confirmed the infectivity and stability of these recombinants in cats.
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U2 - 10.1007/s007050170182
DO - 10.1007/s007050170182
M3 - Article
C2 - 11315645
AN - SCOPUS:0035091224
SN - 0304-8608
VL - 146
SP - 379
EP - 387
JO - Archives of Virology
JF - Archives of Virology
IS - 2
ER -