Further development of a recombinant feline herpesvirus type 1 expressing the Gag protein of feline immunodeficiency virus

E. Sato, T. Miyazawa, Y. Nishimura, Yasuhiro H Ikeda, K. Kawakami, M. Mochizuki, N. Yokoyama, K. Maeda, K. Fujita, M. Kohmoto, E. Takahashi, T. Mikami

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have previously reported the construction of a recombinant feline herpesvirus type 1 (FHV-1), designated C7301ddlTK-gag, expressing the Gag precursor protein of feline immunodeficiency virus (FIV). In this study, we report the construction of a further recombinant FHV-1 (ddlTK(gBp)-gag) which carries an FHV-1 gB promoter sequence upstream of the FIV gag gene of C7301ddlTK-gag. Strong expression of the FIV Gag protein by ddlTK(gBp)-gag was confirmed by immunoblot analyses and enzyme-linked immunosorbent assays. Although C7301ddlTK-gag and ddlTK(gBp)-gag failed to induce anti-FIV Gag antibodies in cats, we confirmed the infectivity and stability of these recombinants in cats.

Original languageEnglish (US)
Pages (from-to)379-387
Number of pages9
JournalArchives of Virology
Volume146
Issue number2
DOIs
StatePublished - 2001
Externally publishedYes

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Feline Immunodeficiency Virus
gag Gene Products
Herpesviridae
Felidae
Cats
gag Genes
Enzyme-Linked Immunosorbent Assay
Antibodies

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

Sato, E., Miyazawa, T., Nishimura, Y., Ikeda, Y. H., Kawakami, K., Mochizuki, M., ... Mikami, T. (2001). Further development of a recombinant feline herpesvirus type 1 expressing the Gag protein of feline immunodeficiency virus. Archives of Virology, 146(2), 379-387. https://doi.org/10.1007/s007050170182

Further development of a recombinant feline herpesvirus type 1 expressing the Gag protein of feline immunodeficiency virus. / Sato, E.; Miyazawa, T.; Nishimura, Y.; Ikeda, Yasuhiro H; Kawakami, K.; Mochizuki, M.; Yokoyama, N.; Maeda, K.; Fujita, K.; Kohmoto, M.; Takahashi, E.; Mikami, T.

In: Archives of Virology, Vol. 146, No. 2, 2001, p. 379-387.

Research output: Contribution to journalArticle

Sato, E, Miyazawa, T, Nishimura, Y, Ikeda, YH, Kawakami, K, Mochizuki, M, Yokoyama, N, Maeda, K, Fujita, K, Kohmoto, M, Takahashi, E & Mikami, T 2001, 'Further development of a recombinant feline herpesvirus type 1 expressing the Gag protein of feline immunodeficiency virus', Archives of Virology, vol. 146, no. 2, pp. 379-387. https://doi.org/10.1007/s007050170182
Sato, E. ; Miyazawa, T. ; Nishimura, Y. ; Ikeda, Yasuhiro H ; Kawakami, K. ; Mochizuki, M. ; Yokoyama, N. ; Maeda, K. ; Fujita, K. ; Kohmoto, M. ; Takahashi, E. ; Mikami, T. / Further development of a recombinant feline herpesvirus type 1 expressing the Gag protein of feline immunodeficiency virus. In: Archives of Virology. 2001 ; Vol. 146, No. 2. pp. 379-387.
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