Fundamental gating mechanism of nicotinic receptor channel revealed by mutation causing a congenital myasthenic syndrome

Hai Long Wang, Kinji Ohno, Margherita Milone, Joan M. Brengman, Amelia Evoli, Anna Paola Batocchi, Lefkos T. Middleton, Kyproula Christodoulou, Andrew G. Engel, Steven M. Sine

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

We describe the genetic and kinetic defects in a congenital myasthenic syndrome due to the mutation εA411P in the amphipathic helix of the acetylcholine receptor (AChR) ε subunit. Myasthenic patients from three unrelated families are either homozygous for εA411P or are heterozygous and harbor a null mutation in the second ε allele, indicating that εA411P is recessive. We expressed human AChRs containing wild-type or A411P ε subunits in 293HEK cells, recorded single channel currents at high bandwidth, and determined microscopic rate constants for individual channels using hidden Markov modeling. For individual wild-type and mutant channels, each rate constant distributes as a Gaussian function, but the spread in the distributions for channel opening and closing rate constants is greatly expanded by εA411P. Prolines engineered into positions flanking residue 411 of the ε subunit greatly increase the range of activation kinetics similar to εA411P, whereas prolines engineered into positions equivalent to εA411 in β and δ subunits are without effect. Thus, the amphipathic helix of the ε subunit stabilizes the channel, minimizing the number and range of kinetic modes accessible to individual AChRs. The findings suggest that analogous stabilizing structures are present in other ion channels, and possibly allosteric proteins in general, and that they evolved to maintain uniformity of activation episodes. The findings further suggest that the fundamental gating mechanism of the AChR channel can be explained by a corrugated energy landscape superimposed on a steeply sloped energy well.

Original languageEnglish (US)
Pages (from-to)449-460
Number of pages12
JournalJournal of General Physiology
Volume116
Issue number3
DOIs
StatePublished - 2000

Keywords

  • Channel gating
  • Congenital myasthenic syndrome
  • Energy landscape
  • Hidden Markov modeling
  • Single channel kinetics

ASJC Scopus subject areas

  • Physiology

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