Functional characterization of mouse spinal cord infiltrating CD8+ lymphocytes

Chandra Deb; Charles L. Howe

doi:10.1016/j.jneuroim.2009.06.013

Functional characterization of mouse spinal cord infiltrating CD8+ lymphocytes

Chandra Deb, Charles L. Howe

Neurology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Understanding the immunopathogenesis of neuroimmunological diseases of the CNS requires a robust method for isolating and characterizing the immune effector cells that infiltrate the spinal cord in animal models. We have developed a simple and rapid isolation method that produces high yields of spinal cord infiltrating leukocytes from a single demyelinated spinal cord and which maintains high surface expression of key immunophenotyping antigens. Using this method and the Theiler's virus model of chronic demyelination, we report the presence of spinal cord infiltrating acute effector CD8⁺ lymphocytes that are CD45^hiCD44^loCD62L^- and a population of spinal cord infiltrating target effector memory CD8⁺ lymphocytes that are CD45^hiCD44^hiCD62L^-. These cells respond robustly to ex vivo stimulation by producing interferon γ but do not exhibit specificity for Theiler's virus in a cytotoxicity assay. We conclude that target-derived lymphocytes in a mouse model of chronic spinal cord demyelination may have unique functional specificities.

Original language	English (US)
Pages (from-to)	33-42
Number of pages	10
Journal	Journal of neuroimmunology
Volume	214
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2009.06.013
State	Published - Sep 29 2009

Keywords

CD8+ T cells
Cell analysis
Flow cytometry
Interferon γ
Multiple sclerosis
qRT-PCR

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/j.jneuroim.2009.06.013

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title = "Functional characterization of mouse spinal cord infiltrating CD8+ lymphocytes",

abstract = "Understanding the immunopathogenesis of neuroimmunological diseases of the CNS requires a robust method for isolating and characterizing the immune effector cells that infiltrate the spinal cord in animal models. We have developed a simple and rapid isolation method that produces high yields of spinal cord infiltrating leukocytes from a single demyelinated spinal cord and which maintains high surface expression of key immunophenotyping antigens. Using this method and the Theiler's virus model of chronic demyelination, we report the presence of spinal cord infiltrating acute effector CD8+ lymphocytes that are CD45hiCD44loCD62L- and a population of spinal cord infiltrating target effector memory CD8+ lymphocytes that are CD45hiCD44hiCD62L-. These cells respond robustly to ex vivo stimulation by producing interferon γ but do not exhibit specificity for Theiler's virus in a cytotoxicity assay. We conclude that target-derived lymphocytes in a mouse model of chronic spinal cord demyelination may have unique functional specificities.",

keywords = "CD8+ T cells, Cell analysis, Flow cytometry, Interferon γ, Multiple sclerosis, qRT-PCR",

author = "Chandra Deb and Howe, {Charles L.}",

note = "Funding Information: This work was funded by a grant from the National MS Society (RG3636), a Mayo early career development award, a Mayo Neuroscience theme career development award, and a generous gift from Donald and Frances Herdrich.",

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AU - Howe, Charles L.

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Y1 - 2009/9/29

N2 - Understanding the immunopathogenesis of neuroimmunological diseases of the CNS requires a robust method for isolating and characterizing the immune effector cells that infiltrate the spinal cord in animal models. We have developed a simple and rapid isolation method that produces high yields of spinal cord infiltrating leukocytes from a single demyelinated spinal cord and which maintains high surface expression of key immunophenotyping antigens. Using this method and the Theiler's virus model of chronic demyelination, we report the presence of spinal cord infiltrating acute effector CD8+ lymphocytes that are CD45hiCD44loCD62L- and a population of spinal cord infiltrating target effector memory CD8+ lymphocytes that are CD45hiCD44hiCD62L-. These cells respond robustly to ex vivo stimulation by producing interferon γ but do not exhibit specificity for Theiler's virus in a cytotoxicity assay. We conclude that target-derived lymphocytes in a mouse model of chronic spinal cord demyelination may have unique functional specificities.

AB - Understanding the immunopathogenesis of neuroimmunological diseases of the CNS requires a robust method for isolating and characterizing the immune effector cells that infiltrate the spinal cord in animal models. We have developed a simple and rapid isolation method that produces high yields of spinal cord infiltrating leukocytes from a single demyelinated spinal cord and which maintains high surface expression of key immunophenotyping antigens. Using this method and the Theiler's virus model of chronic demyelination, we report the presence of spinal cord infiltrating acute effector CD8+ lymphocytes that are CD45hiCD44loCD62L- and a population of spinal cord infiltrating target effector memory CD8+ lymphocytes that are CD45hiCD44hiCD62L-. These cells respond robustly to ex vivo stimulation by producing interferon γ but do not exhibit specificity for Theiler's virus in a cytotoxicity assay. We conclude that target-derived lymphocytes in a mouse model of chronic spinal cord demyelination may have unique functional specificities.

KW - CD8+ T cells

KW - Cell analysis

KW - Flow cytometry

KW - Interferon γ

KW - Multiple sclerosis

KW - qRT-PCR

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Functional characterization of mouse spinal cord infiltrating CD8+ lymphocytes

Abstract

Keywords

ASJC Scopus subject areas

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