Functional characterization of mouse spinal cord infiltrating CD8+ lymphocytes

Chandra Deb, Charles L. Howe

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Understanding the immunopathogenesis of neuroimmunological diseases of the CNS requires a robust method for isolating and characterizing the immune effector cells that infiltrate the spinal cord in animal models. We have developed a simple and rapid isolation method that produces high yields of spinal cord infiltrating leukocytes from a single demyelinated spinal cord and which maintains high surface expression of key immunophenotyping antigens. Using this method and the Theiler's virus model of chronic demyelination, we report the presence of spinal cord infiltrating acute effector CD8+ lymphocytes that are CD45hiCD44loCD62L- and a population of spinal cord infiltrating target effector memory CD8+ lymphocytes that are CD45hiCD44hiCD62L-. These cells respond robustly to ex vivo stimulation by producing interferon γ but do not exhibit specificity for Theiler's virus in a cytotoxicity assay. We conclude that target-derived lymphocytes in a mouse model of chronic spinal cord demyelination may have unique functional specificities.

Original languageEnglish (US)
Pages (from-to)33-42
Number of pages10
JournalJournal of neuroimmunology
Volume214
Issue number1-2
DOIs
StatePublished - Sep 29 2009

Keywords

  • CD8+ T cells
  • Cell analysis
  • Flow cytometry
  • Interferon γ
  • Multiple sclerosis
  • qRT-PCR

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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