TY - JOUR
T1 - Functional changes in nonadrenergic, noncholinergic inhibitory neurons in ileal circular smooth muscle after small bowel transplantation in rats
AU - Shibata, Chikashi
AU - Balsiger, Bruno M.
AU - Anding, William J.
AU - Duenes, Judith A.
AU - Miller, Virginia M.
AU - Sarr, Michael G.
N1 - Funding Information:
Manuscript rece ived Novembe r 20, 1997; acce pted July 31, 1998. From De partment of Surgery and Gastroe nterology Rese arch Unit, Mao Cylinic and Foundation, Rochester, Minnesota. This work was supported by USPHS NIH DK39337, Ethicon Corporation, and the Mao Foyundation. Parts of this work were published in abstract form in Gastroenterology112:A824,1997. Addressforreprintrequests:Dr.MichaelG.Sarr,Gastroenter-Dr. Shibata’s present address: First Department of Surger,yologReysaechrUnit,MaoCylinic,20F0rstSitretSW,Rochesert, TohokuUniversitySchoolofMedicine,Sendai,Japan. Minnesota55905.
PY - 1998
Y1 - 1998
N2 - This experiment was designed to determine mechanisms of change in nonadrenergic, noncholinergic (NANC) inhibitory neurons in the ileum after small bowel transplantation (SBT) in the rat and whether nitric oxide (NO) serves as an important NANC inhibitory neurotransmitter in the rat ileum. Eight groups of rats (N > 8 rats/group) were studied: neurally intact unoperated controls; rats one week after anesthesia and sham celiotomy; and separate groups one and eight weeks after either 40 min of cold ischemia of the jejunoileum, combined jejunal and ileal intestinal transection/reanastomosis, or orthotopic SBT of the entire jejunoileum. Contractile activity was evaluated in fulMhickness ileal circular muscle strips under isometric conditions. Spontaneous activity did not differ among groups. In all groups, exogenous NO, NC-monomethyl-L-arginine (L-NMMA, an NO synthase inhibitor), and methylene blue (soluble guanylate cyclase inhibitor) had no effect on spontaneous activity, while 8-bromocyclic guanosine monophosphate (8Br-cGMP) inhibited contractile activity in all groups. Low frequency (2-10 Hz) electrical field stimulation (EFS) inhibited contractile activity onlv in control and SBT groups; L-NMMA and methylene blue did not alter the response to EFS in any group. These results suggest that each aspect of the SBT procedure, ischemia/reperfusion injury, disruption of enteric neural continuity by intestinal transection, and extrinsic denervation, alter function of enteric ileal inhibitory neurons separately early (one week) after operation. NO, a known inhibitory neurotransmitter in other gut regions, does not affect ileal circular muscle in neurally intact tissue nor mediate functional changes in inhibitory nerve function nor smooth muscle contractility after SBT.
AB - This experiment was designed to determine mechanisms of change in nonadrenergic, noncholinergic (NANC) inhibitory neurons in the ileum after small bowel transplantation (SBT) in the rat and whether nitric oxide (NO) serves as an important NANC inhibitory neurotransmitter in the rat ileum. Eight groups of rats (N > 8 rats/group) were studied: neurally intact unoperated controls; rats one week after anesthesia and sham celiotomy; and separate groups one and eight weeks after either 40 min of cold ischemia of the jejunoileum, combined jejunal and ileal intestinal transection/reanastomosis, or orthotopic SBT of the entire jejunoileum. Contractile activity was evaluated in fulMhickness ileal circular muscle strips under isometric conditions. Spontaneous activity did not differ among groups. In all groups, exogenous NO, NC-monomethyl-L-arginine (L-NMMA, an NO synthase inhibitor), and methylene blue (soluble guanylate cyclase inhibitor) had no effect on spontaneous activity, while 8-bromocyclic guanosine monophosphate (8Br-cGMP) inhibited contractile activity in all groups. Low frequency (2-10 Hz) electrical field stimulation (EFS) inhibited contractile activity onlv in control and SBT groups; L-NMMA and methylene blue did not alter the response to EFS in any group. These results suggest that each aspect of the SBT procedure, ischemia/reperfusion injury, disruption of enteric neural continuity by intestinal transection, and extrinsic denervation, alter function of enteric ileal inhibitory neurons separately early (one week) after operation. NO, a known inhibitory neurotransmitter in other gut regions, does not affect ileal circular muscle in neurally intact tissue nor mediate functional changes in inhibitory nerve function nor smooth muscle contractility after SBT.
KW - Small bowel transplantation; inhibitory neurotransmitter; nitric oxide; small bowel motility; contractile activity
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U2 - 10.1023/A:1026630115009
DO - 10.1023/A:1026630115009
M3 - Article
C2 - 9824132
AN - SCOPUS:0031772877
SN - 0163-2116
VL - 43
SP - 2446
EP - 2454
JO - Digestive diseases and sciences
JF - Digestive diseases and sciences
IS - 11
ER -