Functional Analysis of the Replication Fork Proteome Identifies BET Proteins as PCNA Regulators

Sarah R. Wessel; Kareem N. Mohni; Jessica W. Luzwick; Huzefa Dungrawala; David Cortez

doi:10.1016/j.celrep.2019.08.051

Functional Analysis of the Replication Fork Proteome Identifies BET Proteins as PCNA Regulators

Sarah R. Wessel, Kareem N. Mohni, Jessica W. Luzwick, Huzefa Dungrawala, David Cortez

Molecular Medicine

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Wessel et al. use iPOND purifications and loss-of-function analyses to identify and characterize a replication fork and nascent chromatin proteome of 593 proteins. They demonstrate the resource's utility by showing that the BET proteins BRD2, BRD3, and BRD4 inhibit ATAD5-dependent PCNA unloading from chromatin.

Original language	English (US)
Pages (from-to)	3497-3509.e4
Journal	Cell reports
Volume	28
Issue number	13
DOIs	https://doi.org/10.1016/j.celrep.2019.08.051
State	Published - Sep 24 2019

Keywords

ATAD5
BET domain
DNA replication
PCNA
chromatin
iPOND
proteomics
replisome

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.celrep.2019.08.051

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abstract = "Wessel et al. use iPOND purifications and loss-of-function analyses to identify and characterize a replication fork and nascent chromatin proteome of 593 proteins. They demonstrate the resource's utility by showing that the BET proteins BRD2, BRD3, and BRD4 inhibit ATAD5-dependent PCNA unloading from chromatin.",

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author = "Wessel, {Sarah R.} and Mohni, {Kareem N.} and Luzwick, {Jessica W.} and Huzefa Dungrawala and David Cortez",

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AU - Mohni, Kareem N.

AU - Luzwick, Jessica W.

AU - Dungrawala, Huzefa

AU - Cortez, David

PY - 2019/9/24

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AB - Wessel et al. use iPOND purifications and loss-of-function analyses to identify and characterize a replication fork and nascent chromatin proteome of 593 proteins. They demonstrate the resource's utility by showing that the BET proteins BRD2, BRD3, and BRD4 inhibit ATAD5-dependent PCNA unloading from chromatin.

KW - ATAD5

KW - BET domain

KW - DNA replication

KW - PCNA

KW - chromatin

KW - iPOND

KW - proteomics

KW - replisome

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JO - Cell reports

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ER -

Functional Analysis of the Replication Fork Proteome Identifies BET Proteins as PCNA Regulators

Abstract

Keywords

ASJC Scopus subject areas

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