Functional analysis of human hematopoietic stem cell gene expression using zebrafish

Craig E. Eckfeldt, Eric M. Mendenhall, Catherine M. Flynn, Tzu Fei Wang, Michael A. Pickart, Suzanne M. Grindle, Stephen C Ekker, Catherine M. Verfaillie

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Although several reports have characterized the hematopoietic stem cell (HSC) transcriptome, the roles of HSC-specific genes in hematopoiesis remain elusive. To identify candidate regulators of HSC fate decisions, we compared the transcriptome of human umbilical cord blood and bone marrow CD34 +CD33-CD38-Rholoc-kit+ cells, enriched for hematopoietic stem/progenitor cells with CD34 +CD33-CD38-Rhohi cells, enriched in committed progenitors. We identified 277 differentially expressed transcripts conserved in these ontogenically distinct cell sources. We next performed a morpholino antisense oligonucleotide (MO)-based functional screen in zebrafish to determine the hematopoietic function of 61 genes that had no previously known function in HSC biology and for which a likely zebrafish ortholog could be identified. MO knock down of 14/61 (23%) of the differentially expressed transcripts resulted in hematopoietic defects in developing zebrafish embryos, as demonstrated by altered levels of circulating blood cells at 30 and 48 h postfertilization and subsequently confirmed by quantitative RT-PCR for erythroid-specific hbae1 and myeloid-specific lcp1 transcripts. Recapitulating the knockdown phenotype using a second MO of independent sequence, absence of the phenotype using a mismatched MO sequence, and rescue of the phenotype by cDNA-based overexpression of the targeted transcript for zebrafish spry4 confirmed the specificity of MO targeting in this system. Further characterization of the spry4-deficient zebrafish embryos demonstrated that hematopoietic defects were not due to more widespread defects in the mesodermal development, and therefore represented primary defects in HSC specification, proliferation, and/or differentiation. Overall, this high-throughput screen for the functional validation of differentially expressed genes using a zebrafish model of hematopoiesis represents a major step toward obtaining meaningful information from global gene profiling of HSCs.

Original languageEnglish (US)
JournalPLoS Biology
Volume3
Issue number8
DOIs
StatePublished - Aug 2005
Externally publishedYes

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Functional analysis
Zebrafish
Morpholinos
Hematopoietic Stem Cells
Stem cells
Danio rerio
Gene expression
Antisense Oligonucleotides
oligonucleotides
Gene Expression
gene expression
Genes
Defects
hematopoiesis
phenotype
transcriptome
Hematopoiesis
stem cells
Phenotype
Transcriptome

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Eckfeldt, C. E., Mendenhall, E. M., Flynn, C. M., Wang, T. F., Pickart, M. A., Grindle, S. M., ... Verfaillie, C. M. (2005). Functional analysis of human hematopoietic stem cell gene expression using zebrafish. PLoS Biology, 3(8). https://doi.org/10.1371/journal.pbio.0030254

Functional analysis of human hematopoietic stem cell gene expression using zebrafish. / Eckfeldt, Craig E.; Mendenhall, Eric M.; Flynn, Catherine M.; Wang, Tzu Fei; Pickart, Michael A.; Grindle, Suzanne M.; Ekker, Stephen C; Verfaillie, Catherine M.

In: PLoS Biology, Vol. 3, No. 8, 08.2005.

Research output: Contribution to journalArticle

Eckfeldt, CE, Mendenhall, EM, Flynn, CM, Wang, TF, Pickart, MA, Grindle, SM, Ekker, SC & Verfaillie, CM 2005, 'Functional analysis of human hematopoietic stem cell gene expression using zebrafish', PLoS Biology, vol. 3, no. 8. https://doi.org/10.1371/journal.pbio.0030254
Eckfeldt CE, Mendenhall EM, Flynn CM, Wang TF, Pickart MA, Grindle SM et al. Functional analysis of human hematopoietic stem cell gene expression using zebrafish. PLoS Biology. 2005 Aug;3(8). https://doi.org/10.1371/journal.pbio.0030254
Eckfeldt, Craig E. ; Mendenhall, Eric M. ; Flynn, Catherine M. ; Wang, Tzu Fei ; Pickart, Michael A. ; Grindle, Suzanne M. ; Ekker, Stephen C ; Verfaillie, Catherine M. / Functional analysis of human hematopoietic stem cell gene expression using zebrafish. In: PLoS Biology. 2005 ; Vol. 3, No. 8.
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