Functional alteration of PARL contributes to mitochondrial dysregulation in Parkinson's disease

Guang Shi, Jeffrey R. Lee, David A. Grimes, Lemuel Racacho, David Ye, Howard Yang, Owen A Ross, Matthew Farrer, G. Angus McQuibban, Dennis E. Bulman

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Molecular genetics has linked mitochondrial dysfunction to the pathogenesis of Parkinson's disease by the discovery of rare, inherited mutations in gene products that associate with the mitochondria. Mutations in PTEN-induced kinase-1 (PINK1), which encodes a mitochondrial kinase, and PARKIN, encoding an E3 ubiquitin ligase, are the most frequent causes of recessive Parkinson's disease. Recent functional studies have revealed that PINK1 recruits PARKIN to mitochondria to initiate mitophagy, an important autophagic quality control mechanism that rids the cell of damaged mitochondria. PINK1 is post-translationally processed into a cleaved form whose levels are tightly regulated, although the significance of this processing is unknown. Here we demonstrate that the mitochondrial protease presenilin-associated rhomboid-like (PARL) can affect the proteolytic processing of PINK1 and that normal PINK1 localization and stability requires PARL's catalytic activity. PARL deficiency impairs PARKIN recruitment to mitochondria, suggesting PINK1's processing and localization are important in determining its interaction with PARKIN. We sequenced the PARL gene in Parkinson's disease patients and discovered a novel missense mutation in a functional domain of PARL's N-terminus. This PARL mutant is not sufficient to rescue PARKIN recruitment, suggesting that impaired mitophagy may be an underlying mechanism of disease pathogenesis in patients with PARL mutations.

Original languageEnglish (US)
Article numberddr077
Pages (from-to)1966-1974
Number of pages9
JournalHuman Molecular Genetics
Volume20
Issue number10
DOIs
StatePublished - May 2011

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Presenilins
Parkinson Disease
Phosphotransferases
Mitochondria
Mitochondrial Degradation
Mutation
Ubiquitin-Protein Ligases
Missense Mutation
Quality Control
Genes
Molecular Biology
Peptide Hydrolases

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Molecular Biology

Cite this

Shi, G., Lee, J. R., Grimes, D. A., Racacho, L., Ye, D., Yang, H., ... Bulman, D. E. (2011). Functional alteration of PARL contributes to mitochondrial dysregulation in Parkinson's disease. Human Molecular Genetics, 20(10), 1966-1974. [ddr077]. https://doi.org/10.1093/hmg/ddr077

Functional alteration of PARL contributes to mitochondrial dysregulation in Parkinson's disease. / Shi, Guang; Lee, Jeffrey R.; Grimes, David A.; Racacho, Lemuel; Ye, David; Yang, Howard; Ross, Owen A; Farrer, Matthew; McQuibban, G. Angus; Bulman, Dennis E.

In: Human Molecular Genetics, Vol. 20, No. 10, ddr077, 05.2011, p. 1966-1974.

Research output: Contribution to journalArticle

Shi, G, Lee, JR, Grimes, DA, Racacho, L, Ye, D, Yang, H, Ross, OA, Farrer, M, McQuibban, GA & Bulman, DE 2011, 'Functional alteration of PARL contributes to mitochondrial dysregulation in Parkinson's disease', Human Molecular Genetics, vol. 20, no. 10, ddr077, pp. 1966-1974. https://doi.org/10.1093/hmg/ddr077
Shi, Guang ; Lee, Jeffrey R. ; Grimes, David A. ; Racacho, Lemuel ; Ye, David ; Yang, Howard ; Ross, Owen A ; Farrer, Matthew ; McQuibban, G. Angus ; Bulman, Dennis E. / Functional alteration of PARL contributes to mitochondrial dysregulation in Parkinson's disease. In: Human Molecular Genetics. 2011 ; Vol. 20, No. 10. pp. 1966-1974.
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