Frequent loss of heterozygosity on chromosome arm 18q in squamous cell carcinemas: Identification of 2 regions of loss-18q11.1-q12.3 and 18q21.1- q23

Jessica W. Jones, Jeffrey R. Raval, Theodore F. Beals, Maria J. Worsham, Daniel L. Van Dyke, Ramon M. Esclamado, Gregory T. Wolf, Carol R. Bradford, Tamara Miller, Thomas E. Carey

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Objectives: To determine the frequency and regions of loss on chromosome arm 18q in uncultured head and neck squamous cell carcinomas. Design: Polymerase chain reaction amplification of DNA extracted from 18 tumor specimens (1 patient had 2 tumors) and blood samples from 17 patients with head and neck squamous cell carcinoma was performed using primers flanking 16 microsatellite repeat polymorphisms spanning most of chromosome 18q. DNA was extracted only from specimens with greater than 70% tumor nuclei. Setting: Research university. Patients: Seventeen individuals with newly diagnosed head and neck cancer. Main Outcome Measure: Loss of heterozygosity (LOH). Results: There was LOH at more than I locus in 52% (9/17) of the tumors; 3 tumors had LOH at all informative markers. Four had loss at only 1 locus, raising the total with loss to 12 (75%) of 16. Loss of 18ql 1.1-q12.3 in 4 tumors without distal loss defines a proximal region of loss. Loss of heterozygosity affecting 18q21.1 in 1 tumor, without proximal loss and LOH for 18q21.1, 18q22, or 18q23 in 9 (52%) of 17 tumors defines a distal region of loss. Conclusions: Loss of heterozygosity on chromosome arm 18q is not an artifact of in vitro culture. The finding of 18q LOH in 50% to 70% tumors makes 18q an important region for study. Regions 18ql 1.1-ql 2.3 and 18q21.1q23 are common regions of loss, indicating that there may be more than one 18q tumor suppressor gene involved in the genesis and progression of head and neck squamous cell carcinomas.

Original languageEnglish (US)
Pages (from-to)610-614
Number of pages5
JournalArchives of Otolaryngology - Head and Neck Surgery
Volume123
Issue number6
StatePublished - Jun 1997
Externally publishedYes

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Loss of Heterozygosity
Chromosomes
Epithelial Cells
Neoplasms
DNA
Head and Neck Neoplasms
Tumor Suppressor Genes
Microsatellite Repeats
Artifacts
Outcome Assessment (Health Care)
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Jones, J. W., Raval, J. R., Beals, T. F., Worsham, M. J., Van Dyke, D. L., Esclamado, R. M., ... Carey, T. E. (1997). Frequent loss of heterozygosity on chromosome arm 18q in squamous cell carcinemas: Identification of 2 regions of loss-18q11.1-q12.3 and 18q21.1- q23. Archives of Otolaryngology - Head and Neck Surgery, 123(6), 610-614.

Frequent loss of heterozygosity on chromosome arm 18q in squamous cell carcinemas : Identification of 2 regions of loss-18q11.1-q12.3 and 18q21.1- q23. / Jones, Jessica W.; Raval, Jeffrey R.; Beals, Theodore F.; Worsham, Maria J.; Van Dyke, Daniel L.; Esclamado, Ramon M.; Wolf, Gregory T.; Bradford, Carol R.; Miller, Tamara; Carey, Thomas E.

In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 123, No. 6, 06.1997, p. 610-614.

Research output: Contribution to journalArticle

Jones, JW, Raval, JR, Beals, TF, Worsham, MJ, Van Dyke, DL, Esclamado, RM, Wolf, GT, Bradford, CR, Miller, T & Carey, TE 1997, 'Frequent loss of heterozygosity on chromosome arm 18q in squamous cell carcinemas: Identification of 2 regions of loss-18q11.1-q12.3 and 18q21.1- q23', Archives of Otolaryngology - Head and Neck Surgery, vol. 123, no. 6, pp. 610-614.
Jones, Jessica W. ; Raval, Jeffrey R. ; Beals, Theodore F. ; Worsham, Maria J. ; Van Dyke, Daniel L. ; Esclamado, Ramon M. ; Wolf, Gregory T. ; Bradford, Carol R. ; Miller, Tamara ; Carey, Thomas E. / Frequent loss of heterozygosity on chromosome arm 18q in squamous cell carcinemas : Identification of 2 regions of loss-18q11.1-q12.3 and 18q21.1- q23. In: Archives of Otolaryngology - Head and Neck Surgery. 1997 ; Vol. 123, No. 6. pp. 610-614.
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title = "Frequent loss of heterozygosity on chromosome arm 18q in squamous cell carcinemas: Identification of 2 regions of loss-18q11.1-q12.3 and 18q21.1- q23",
abstract = "Objectives: To determine the frequency and regions of loss on chromosome arm 18q in uncultured head and neck squamous cell carcinomas. Design: Polymerase chain reaction amplification of DNA extracted from 18 tumor specimens (1 patient had 2 tumors) and blood samples from 17 patients with head and neck squamous cell carcinoma was performed using primers flanking 16 microsatellite repeat polymorphisms spanning most of chromosome 18q. DNA was extracted only from specimens with greater than 70{\%} tumor nuclei. Setting: Research university. Patients: Seventeen individuals with newly diagnosed head and neck cancer. Main Outcome Measure: Loss of heterozygosity (LOH). Results: There was LOH at more than I locus in 52{\%} (9/17) of the tumors; 3 tumors had LOH at all informative markers. Four had loss at only 1 locus, raising the total with loss to 12 (75{\%}) of 16. Loss of 18ql 1.1-q12.3 in 4 tumors without distal loss defines a proximal region of loss. Loss of heterozygosity affecting 18q21.1 in 1 tumor, without proximal loss and LOH for 18q21.1, 18q22, or 18q23 in 9 (52{\%}) of 17 tumors defines a distal region of loss. Conclusions: Loss of heterozygosity on chromosome arm 18q is not an artifact of in vitro culture. The finding of 18q LOH in 50{\%} to 70{\%} tumors makes 18q an important region for study. Regions 18ql 1.1-ql 2.3 and 18q21.1q23 are common regions of loss, indicating that there may be more than one 18q tumor suppressor gene involved in the genesis and progression of head and neck squamous cell carcinomas.",
author = "Jones, {Jessica W.} and Raval, {Jeffrey R.} and Beals, {Theodore F.} and Worsham, {Maria J.} and {Van Dyke}, {Daniel L.} and Esclamado, {Ramon M.} and Wolf, {Gregory T.} and Bradford, {Carol R.} and Tamara Miller and Carey, {Thomas E.}",
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T1 - Frequent loss of heterozygosity on chromosome arm 18q in squamous cell carcinemas

T2 - Identification of 2 regions of loss-18q11.1-q12.3 and 18q21.1- q23

AU - Jones, Jessica W.

AU - Raval, Jeffrey R.

AU - Beals, Theodore F.

AU - Worsham, Maria J.

AU - Van Dyke, Daniel L.

AU - Esclamado, Ramon M.

AU - Wolf, Gregory T.

AU - Bradford, Carol R.

AU - Miller, Tamara

AU - Carey, Thomas E.

PY - 1997/6

Y1 - 1997/6

N2 - Objectives: To determine the frequency and regions of loss on chromosome arm 18q in uncultured head and neck squamous cell carcinomas. Design: Polymerase chain reaction amplification of DNA extracted from 18 tumor specimens (1 patient had 2 tumors) and blood samples from 17 patients with head and neck squamous cell carcinoma was performed using primers flanking 16 microsatellite repeat polymorphisms spanning most of chromosome 18q. DNA was extracted only from specimens with greater than 70% tumor nuclei. Setting: Research university. Patients: Seventeen individuals with newly diagnosed head and neck cancer. Main Outcome Measure: Loss of heterozygosity (LOH). Results: There was LOH at more than I locus in 52% (9/17) of the tumors; 3 tumors had LOH at all informative markers. Four had loss at only 1 locus, raising the total with loss to 12 (75%) of 16. Loss of 18ql 1.1-q12.3 in 4 tumors without distal loss defines a proximal region of loss. Loss of heterozygosity affecting 18q21.1 in 1 tumor, without proximal loss and LOH for 18q21.1, 18q22, or 18q23 in 9 (52%) of 17 tumors defines a distal region of loss. Conclusions: Loss of heterozygosity on chromosome arm 18q is not an artifact of in vitro culture. The finding of 18q LOH in 50% to 70% tumors makes 18q an important region for study. Regions 18ql 1.1-ql 2.3 and 18q21.1q23 are common regions of loss, indicating that there may be more than one 18q tumor suppressor gene involved in the genesis and progression of head and neck squamous cell carcinomas.

AB - Objectives: To determine the frequency and regions of loss on chromosome arm 18q in uncultured head and neck squamous cell carcinomas. Design: Polymerase chain reaction amplification of DNA extracted from 18 tumor specimens (1 patient had 2 tumors) and blood samples from 17 patients with head and neck squamous cell carcinoma was performed using primers flanking 16 microsatellite repeat polymorphisms spanning most of chromosome 18q. DNA was extracted only from specimens with greater than 70% tumor nuclei. Setting: Research university. Patients: Seventeen individuals with newly diagnosed head and neck cancer. Main Outcome Measure: Loss of heterozygosity (LOH). Results: There was LOH at more than I locus in 52% (9/17) of the tumors; 3 tumors had LOH at all informative markers. Four had loss at only 1 locus, raising the total with loss to 12 (75%) of 16. Loss of 18ql 1.1-q12.3 in 4 tumors without distal loss defines a proximal region of loss. Loss of heterozygosity affecting 18q21.1 in 1 tumor, without proximal loss and LOH for 18q21.1, 18q22, or 18q23 in 9 (52%) of 17 tumors defines a distal region of loss. Conclusions: Loss of heterozygosity on chromosome arm 18q is not an artifact of in vitro culture. The finding of 18q LOH in 50% to 70% tumors makes 18q an important region for study. Regions 18ql 1.1-ql 2.3 and 18q21.1q23 are common regions of loss, indicating that there may be more than one 18q tumor suppressor gene involved in the genesis and progression of head and neck squamous cell carcinomas.

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