Frequent inactivation of PTEN/MMAC1 in primary prostate cancer

Paul Cairns, Kenji Okami, Sarel Halachmi, Naomi Halachmi, Manel Esteller, James G. Herman, Jin Jen, W. B. Isaacs, G. Steven Bova, David Sidransky

Research output: Contribution to journalArticle

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Abstract

Sporadic prostate carcinoma is the most common male cancer in the Western world, yet many of the major genetic events involved in the progression of this often fatal cancer remain to be elucidated. Numerous cytogenetic and allelotype studies have reported frequent loss of heterozygosity on chromosomal arm 10q in sporadic prostate cancer. Deletion mapping studies have unambiguously identified a region of chromosome 10q23 to be the minimal area of loss. A new tumor suppressor gene, PTEN/MMAC1, was isolated recently at this region of chromosome 10q23 and found to be inactivated by mutation in three prostate cancer cell lines. We screened 80 prostate tumors by microsatellite analysis and found chromosome 10q23 to be deleted in 23 cases. We then proceeded with sequence analysis of the entire PTEN/MMAC1 coding region and tested for homozygous deletion with new intragenic markers in these 23 cases with 10q23 loss of heterozygosity. The identification of the second mutational event in 10 (43%) tumors establishes PTEN/MMAC1 as a main inactivation target of 10q loss in sporadic prostate cancer.

Original languageEnglish (US)
Pages (from-to)4997-5000
Number of pages4
JournalCancer Research
Volume57
Issue number22
StatePublished - Nov 15 1997
Externally publishedYes

Fingerprint

Prostatic Neoplasms
Chromosomes
Loss of Heterozygosity
Prostate
Neoplasms
Western World
Tumor Suppressor Genes
Cytogenetics
Microsatellite Repeats
Sequence Analysis
Carcinoma
Cell Line
Mutation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Cairns, P., Okami, K., Halachmi, S., Halachmi, N., Esteller, M., Herman, J. G., ... Sidransky, D. (1997). Frequent inactivation of PTEN/MMAC1 in primary prostate cancer. Cancer Research, 57(22), 4997-5000.

Frequent inactivation of PTEN/MMAC1 in primary prostate cancer. / Cairns, Paul; Okami, Kenji; Halachmi, Sarel; Halachmi, Naomi; Esteller, Manel; Herman, James G.; Jen, Jin; Isaacs, W. B.; Bova, G. Steven; Sidransky, David.

In: Cancer Research, Vol. 57, No. 22, 15.11.1997, p. 4997-5000.

Research output: Contribution to journalArticle

Cairns, P, Okami, K, Halachmi, S, Halachmi, N, Esteller, M, Herman, JG, Jen, J, Isaacs, WB, Bova, GS & Sidransky, D 1997, 'Frequent inactivation of PTEN/MMAC1 in primary prostate cancer', Cancer Research, vol. 57, no. 22, pp. 4997-5000.
Cairns P, Okami K, Halachmi S, Halachmi N, Esteller M, Herman JG et al. Frequent inactivation of PTEN/MMAC1 in primary prostate cancer. Cancer Research. 1997 Nov 15;57(22):4997-5000.
Cairns, Paul ; Okami, Kenji ; Halachmi, Sarel ; Halachmi, Naomi ; Esteller, Manel ; Herman, James G. ; Jen, Jin ; Isaacs, W. B. ; Bova, G. Steven ; Sidransky, David. / Frequent inactivation of PTEN/MMAC1 in primary prostate cancer. In: Cancer Research. 1997 ; Vol. 57, No. 22. pp. 4997-5000.
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