Frequent homozygous deletions in the FRA3B region in tumor cell lines still leave the FHIT exons intact

Liang Wang, John Darling, Jin San Zhang, Chi Ping Qian, Lynn Hartmann, Cheryl A Conover, Robert Brian Jenkins, David I Smith

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

FRA3B at human chromosomal band 3P14.2 is the most active common fragile site in the human genome. The molecular mechanism of fragility at this region remains unknown but does not involve expansion of a trinucleotide or minisatellite repeat as has been observed for several of the cloned rare fragile sites. Deletions and rearrangements at FRA3B have been observed in a number of distinct tumors. The recently identified putative tumor suppressor gene FHIT spans FRA3B, and various groups have reported identifying deletions in this gene in different tumors. Using a high density of PCR amplifiable markers within FRA3B searching for deletions in the FRA3B region, we have analysed 21 tumor cell lines derived from renal cell, pancreatic, and ovarian carcinomas. We found a commonly deleted region in the renal cell and ovarian carcinoma cell lines located in the middle of an HPV16 viral integration site. Despite the presence of deletions in the FRA3B region in most of the cell lines, we did not detect alterations in FHIT exons in any of the cell lines examined. Thus, deletions of 3p14.2 in these carcinoma cell lines may simply reflect instability of the FRA3B region during tumor progression.

Original languageEnglish (US)
Pages (from-to)635-642
Number of pages8
JournalOncogene
Volume16
Issue number5
StatePublished - Feb 5 1998

Fingerprint

Tumor Cell Line
Exons
Cell Line
Virus Integration
Trinucleotide Repeats
Neoplasms
Minisatellite Repeats
Gene Deletion
Human Genome
Tumor Suppressor Genes
Renal Cell Carcinoma
Carcinoma
Kidney
Polymerase Chain Reaction

Keywords

  • Deletions
  • FHIT
  • FRA3B
  • Tumor cell lines

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Frequent homozygous deletions in the FRA3B region in tumor cell lines still leave the FHIT exons intact. / Wang, Liang; Darling, John; Zhang, Jin San; Qian, Chi Ping; Hartmann, Lynn; Conover, Cheryl A; Jenkins, Robert Brian; Smith, David I.

In: Oncogene, Vol. 16, No. 5, 05.02.1998, p. 635-642.

Research output: Contribution to journalArticle

Wang L, Darling J, Zhang JS, Qian CP, Hartmann L, Conover CA et al. Frequent homozygous deletions in the FRA3B region in tumor cell lines still leave the FHIT exons intact. Oncogene. 1998 Feb 5;16(5):635-642.
Wang, Liang ; Darling, John ; Zhang, Jin San ; Qian, Chi Ping ; Hartmann, Lynn ; Conover, Cheryl A ; Jenkins, Robert Brian ; Smith, David I. / Frequent homozygous deletions in the FRA3B region in tumor cell lines still leave the FHIT exons intact. In: Oncogene. 1998 ; Vol. 16, No. 5. pp. 635-642.
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