Frequent breakpoints in the 3p14.2 fragile site, FRA3B, in pancreatic tumors

Ravi Shridhar, Viji Shridhar, Xiaohong Wang, William Paradee, Michael Dugan, Fazlul Sarkar, Charles Wilke, Thomas W. Glover, Vainutis K. Vaitkevicius, David I. Smith

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

FRA3B, at chromosomal hand 3p14.2, is the most active common fragile site in the human genome. Homozygous deletions in the region of FRA3B have been observed in many types of solid tumors. Recently, the FHIT gene was reported to span FRA3B and was shown to be homozygously deleted in several gastric and colonic tumor cell lines. Several microsatellite markers that precisely define the 1.0-Mb region surrounding FRA3B and FHIT have been utilized, along with other 3p microsatellites, to analyze the loss of 3p sequences in 25 primary pancreatic adenocarcinomas. The high density of microsatellite markers in the 3p 14.2 region enabled us to both identify losses within and flanking FRA3B in pancreatic cancer and define the breakpoints. We observed loss of heterozygosity of 3pl4.2 markers in 16 of 25 pancreatic tumors and loss of heterozygosity of 3p markers outside of 3pl4.2 in only 2 of 25 tumors of this type. There appears to be a dramatic clustering of chromosomal breakpoints at 3pl4.2 in and immediately distal to FRA3B in pancreatic cancer. We detected no homozygous deletions in this region.

Original languageEnglish (US)
Pages (from-to)4347-4350
Number of pages4
JournalCancer research
Volume56
Issue number19
StatePublished - Oct 1 1996

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Shridhar, R., Shridhar, V., Wang, X., Paradee, W., Dugan, M., Sarkar, F., Wilke, C., Glover, T. W., Vaitkevicius, V. K., & Smith, D. I. (1996). Frequent breakpoints in the 3p14.2 fragile site, FRA3B, in pancreatic tumors. Cancer research, 56(19), 4347-4350.