Frequency of loss of hMLH1 expression in colorectal carcinoma increases with advancing age

Sanjay Kakar, Lawrence J. Burgart, Stephen N Thibodeau, Kari G. Rabe, Gloria M Petersen, Richard M. Goldberg, Noralane Morey Lindor

Research output: Contribution to journalArticle

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Abstract

BACKGROUND. The correlation between age at diagnosis and loss of expression of hMLH1 protein in patients with colorectal carcinoma (CRC) has not been evaluated systematically. METHODS. Immunohistochemistry was performed for hMLH1 protein in tumor samples from 867 patients with CRC. The authors defined tumors arising in the cecum, ascending colon, and transverse colon as right-sided and tumors arising in the descending colon, sigmoid colon, and rectum as left-sided. Patients' gender, tumor location (side), and hMLH1 expression were analyzed by age groups. RESULTS. The percentage of tumors with hMLH1 expression loss increased significantly with advancing age (P < 0.0001): There were no tumors in patients age < 40 years that manifested loss of hMLH1 expression, compared with 29% of tumors that manifested loss of hMLH1 expression in patients age > 90 years. Loss of hMLH1 expression occurred more often in patients with right-sided tumors (32.7% vs. 5.2% of patients with left-sided tumors; P < 0.0001) and in tumors from female patients (24.3% vs. 11.5% of tumors from male patients; P < 0.0001). There was no evidence of interaction between gender and tumor location. CONCLUSIONS. Loss of hMLH1 expression in patients with CRC was associated strongly with increasing age. hMLH1 expression loss was more pronounced in tumors from female patients and in tumors that originated on the right side of the colon. Loss of hMLH1 expression in right-sided tumors occurred in nearly 50% of patients age > 90 years. This age-related trend also was observed for males and in tumors that originated in the left colon.

Original languageEnglish (US)
Pages (from-to)1421-1427
Number of pages7
JournalCancer
Volume97
Issue number6
DOIs
StatePublished - Mar 15 2003

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Colorectal Neoplasms
Neoplasms
Descending Colon
Ascending Colon
Transverse Colon
Cecum
Patient Rights
Sigmoid Colon
Rectum
Colon
Proteins
Age Groups
Immunohistochemistry

Keywords

  • Age
  • Colon carcinoma
  • HMLH1
  • Immunohistochemistry
  • Methylation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Frequency of loss of hMLH1 expression in colorectal carcinoma increases with advancing age. / Kakar, Sanjay; Burgart, Lawrence J.; Thibodeau, Stephen N; Rabe, Kari G.; Petersen, Gloria M; Goldberg, Richard M.; Lindor, Noralane Morey.

In: Cancer, Vol. 97, No. 6, 15.03.2003, p. 1421-1427.

Research output: Contribution to journalArticle

Kakar, Sanjay ; Burgart, Lawrence J. ; Thibodeau, Stephen N ; Rabe, Kari G. ; Petersen, Gloria M ; Goldberg, Richard M. ; Lindor, Noralane Morey. / Frequency of loss of hMLH1 expression in colorectal carcinoma increases with advancing age. In: Cancer. 2003 ; Vol. 97, No. 6. pp. 1421-1427.
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title = "Frequency of loss of hMLH1 expression in colorectal carcinoma increases with advancing age",
abstract = "BACKGROUND. The correlation between age at diagnosis and loss of expression of hMLH1 protein in patients with colorectal carcinoma (CRC) has not been evaluated systematically. METHODS. Immunohistochemistry was performed for hMLH1 protein in tumor samples from 867 patients with CRC. The authors defined tumors arising in the cecum, ascending colon, and transverse colon as right-sided and tumors arising in the descending colon, sigmoid colon, and rectum as left-sided. Patients' gender, tumor location (side), and hMLH1 expression were analyzed by age groups. RESULTS. The percentage of tumors with hMLH1 expression loss increased significantly with advancing age (P < 0.0001): There were no tumors in patients age < 40 years that manifested loss of hMLH1 expression, compared with 29{\%} of tumors that manifested loss of hMLH1 expression in patients age > 90 years. Loss of hMLH1 expression occurred more often in patients with right-sided tumors (32.7{\%} vs. 5.2{\%} of patients with left-sided tumors; P < 0.0001) and in tumors from female patients (24.3{\%} vs. 11.5{\%} of tumors from male patients; P < 0.0001). There was no evidence of interaction between gender and tumor location. CONCLUSIONS. Loss of hMLH1 expression in patients with CRC was associated strongly with increasing age. hMLH1 expression loss was more pronounced in tumors from female patients and in tumors that originated on the right side of the colon. Loss of hMLH1 expression in right-sided tumors occurred in nearly 50{\%} of patients age > 90 years. This age-related trend also was observed for males and in tumors that originated in the left colon.",
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T1 - Frequency of loss of hMLH1 expression in colorectal carcinoma increases with advancing age

AU - Kakar, Sanjay

AU - Burgart, Lawrence J.

AU - Thibodeau, Stephen N

AU - Rabe, Kari G.

AU - Petersen, Gloria M

AU - Goldberg, Richard M.

AU - Lindor, Noralane Morey

PY - 2003/3/15

Y1 - 2003/3/15

N2 - BACKGROUND. The correlation between age at diagnosis and loss of expression of hMLH1 protein in patients with colorectal carcinoma (CRC) has not been evaluated systematically. METHODS. Immunohistochemistry was performed for hMLH1 protein in tumor samples from 867 patients with CRC. The authors defined tumors arising in the cecum, ascending colon, and transverse colon as right-sided and tumors arising in the descending colon, sigmoid colon, and rectum as left-sided. Patients' gender, tumor location (side), and hMLH1 expression were analyzed by age groups. RESULTS. The percentage of tumors with hMLH1 expression loss increased significantly with advancing age (P < 0.0001): There were no tumors in patients age < 40 years that manifested loss of hMLH1 expression, compared with 29% of tumors that manifested loss of hMLH1 expression in patients age > 90 years. Loss of hMLH1 expression occurred more often in patients with right-sided tumors (32.7% vs. 5.2% of patients with left-sided tumors; P < 0.0001) and in tumors from female patients (24.3% vs. 11.5% of tumors from male patients; P < 0.0001). There was no evidence of interaction between gender and tumor location. CONCLUSIONS. Loss of hMLH1 expression in patients with CRC was associated strongly with increasing age. hMLH1 expression loss was more pronounced in tumors from female patients and in tumors that originated on the right side of the colon. Loss of hMLH1 expression in right-sided tumors occurred in nearly 50% of patients age > 90 years. This age-related trend also was observed for males and in tumors that originated in the left colon.

AB - BACKGROUND. The correlation between age at diagnosis and loss of expression of hMLH1 protein in patients with colorectal carcinoma (CRC) has not been evaluated systematically. METHODS. Immunohistochemistry was performed for hMLH1 protein in tumor samples from 867 patients with CRC. The authors defined tumors arising in the cecum, ascending colon, and transverse colon as right-sided and tumors arising in the descending colon, sigmoid colon, and rectum as left-sided. Patients' gender, tumor location (side), and hMLH1 expression were analyzed by age groups. RESULTS. The percentage of tumors with hMLH1 expression loss increased significantly with advancing age (P < 0.0001): There were no tumors in patients age < 40 years that manifested loss of hMLH1 expression, compared with 29% of tumors that manifested loss of hMLH1 expression in patients age > 90 years. Loss of hMLH1 expression occurred more often in patients with right-sided tumors (32.7% vs. 5.2% of patients with left-sided tumors; P < 0.0001) and in tumors from female patients (24.3% vs. 11.5% of tumors from male patients; P < 0.0001). There was no evidence of interaction between gender and tumor location. CONCLUSIONS. Loss of hMLH1 expression in patients with CRC was associated strongly with increasing age. hMLH1 expression loss was more pronounced in tumors from female patients and in tumors that originated on the right side of the colon. Loss of hMLH1 expression in right-sided tumors occurred in nearly 50% of patients age > 90 years. This age-related trend also was observed for males and in tumors that originated in the left colon.

KW - Age

KW - Colon carcinoma

KW - HMLH1

KW - Immunohistochemistry

KW - Methylation

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DO - 10.1002/cncr.11206

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