Frequency-dependent lipid raft uptake at rat diaphragm muscle axon terminals

Maria A. Gonzalez Porras, Matthew J. Fogarty, Heather M. Gransee, Gary C Sieck, Carlos Bernardo Mantilla

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Introduction: In motor neurons, cholera toxin B (CTB) binds to the cell-surface ganglioside GM1 and is internalized and transported via structurally unique components of plasma membranes (lipid rafts). Methods: Lipid raft uptake by axon terminals adjoining type-identified rat diaphragm muscle fibers was investigated using CTB and confocal imaging. Results: Lipid raft uptake increased significantly at higher frequency stimulation (80 Hz), compared with lower frequency (20 Hz) and unstimulated (0 Hz) conditions. The fraction of axon terminal occupied by CTB was ∼45% at 0- or 20-Hz stimulation, and increased to ∼65% at 80 Hz. Total CTB fluorescence intensity also increased (∼20%) after 80-Hz stimulation compared with 0 Hz. Discussion: Evidence of increased lipid raft uptake at high stimulation frequencies supports an important role for lipid raft signaling at rat diaphragm muscle axon terminals, primarily for motor units physiologically activated at the higher frequencies. Muscle Nerve, 2019.

Original languageEnglish (US)
JournalMuscle and Nerve
DOIs
StatePublished - Jan 1 2019

Fingerprint

Cholera Toxin
Presynaptic Terminals
Diaphragm
Lipids
Muscles
G(M1) Ganglioside
Motor Neurons
Membrane Lipids
Fluorescence
Cell Membrane

Keywords

  • bulk endocytosis
  • cholera toxin
  • clathrin
  • motor neuron
  • neuromuscular junction
  • synaptic vesicle retrieval

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)

Cite this

Frequency-dependent lipid raft uptake at rat diaphragm muscle axon terminals. / Gonzalez Porras, Maria A.; Fogarty, Matthew J.; Gransee, Heather M.; Sieck, Gary C; Mantilla, Carlos Bernardo.

In: Muscle and Nerve, 01.01.2019.

Research output: Contribution to journalArticle

Gonzalez Porras, Maria A. ; Fogarty, Matthew J. ; Gransee, Heather M. ; Sieck, Gary C ; Mantilla, Carlos Bernardo. / Frequency-dependent lipid raft uptake at rat diaphragm muscle axon terminals. In: Muscle and Nerve. 2019.
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