TY - JOUR
T1 - Freeze-fracture electronmicroscopic analysis of plasma membranes of cultured muscle cells in duchenne dystrophy
AU - Osame, Mitsuhiro
AU - Engel, Andrew G.
AU - Rebouche, Charles J.
AU - Scott, Robert E.
PY - 1981/8
Y1 - 1981/8
N2 - Depletion of intramembranous particles has been reported in the muscle fiber plasma membrane in Duchenne dystrophy. We searched for a similar abnormality in plasma membranes of cultured muscle cells obtained from six patients with Duchenne dystrophy and six controls. A precise method for phase microscopic to freeze-fracture electronmicroscopic correlation was devised to identify the cell of origin of each replicated membrane face. For both control and patient cells, P-face particle density was higher in myotubes than in mononuclear cells. However, there were no significant differences between control and dystrophic cells when comparing P faces of myotubes, E faces of myotubes, P faces of single cells, or E faces of single cells. The frequency distribution of particle diameters was similar in P faces of control and Duchenne myotubes. Other structural features of the freeze-fractured plasma membrane also were similar.
AB - Depletion of intramembranous particles has been reported in the muscle fiber plasma membrane in Duchenne dystrophy. We searched for a similar abnormality in plasma membranes of cultured muscle cells obtained from six patients with Duchenne dystrophy and six controls. A precise method for phase microscopic to freeze-fracture electronmicroscopic correlation was devised to identify the cell of origin of each replicated membrane face. For both control and patient cells, P-face particle density was higher in myotubes than in mononuclear cells. However, there were no significant differences between control and dystrophic cells when comparing P faces of myotubes, E faces of myotubes, P faces of single cells, or E faces of single cells. The frequency distribution of particle diameters was similar in P faces of control and Duchenne myotubes. Other structural features of the freeze-fractured plasma membrane also were similar.
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U2 - 10.1212/wnl.31.8.972
DO - 10.1212/wnl.31.8.972
M3 - Article
C2 - 7196524
AN - SCOPUS:0019412624
VL - 31
SP - 972
EP - 979
JO - Neurology
JF - Neurology
SN - 0028-3878
IS - 8
ER -